Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena, Germany.
Ghent University, Department of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
Methods Mol Biol. 2022;2505:281-291. doi: 10.1007/978-1-0716-2349-7_20.
Transient transformation methods are frequently used to determine gene function. However, until recently only a few methods have been available in the model medicinal plant Catharanthus roseus. Here, we describe a rapid and highly reproducible protocol for the overexpression of genes of interest by agroinfiltration of C. roseus flower petals. This high throughput method is particularly suitable for screening purposes, for instance, target gene screening of transcription factor candidates, and complements other available methods.
瞬时转化方法常用于确定基因功能。然而，直到最近，模式药用植物长春花中才仅有少数几种方法可用。在这里，我们描述了通过农杆菌浸润长春花花瓣来高效表达目的基因的快速且高度可重复的方案。这种高通量方法特别适合筛选目的，例如，筛选转录因子候选物的靶基因，并补充了其他可用的方法。
