Abd El Gayed Eman Masoud, Abo Shady Heba Maged, Elhelbawy Mohammed, El Deen Arafat Eman S
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menoufia University, Egypt.
Department of Gynecology & Obstetrics Department, Faculty of Medicine, Menoufia University, Egypt.
Biochem Biophys Rep. 2022 Jun 14;31:101294. doi: 10.1016/j.bbrep.2022.101294. eCollection 2022 Sep.
Maternal and perinatal mortality is caused by a variety of factors, including preeclampsia. PE's onset and progression may be influenced by lncRNAs. The effect of long non-coding RNA (lncRNA) Colon cancer-associated transcription factor 1 (CCAT1) expression in the placenta of preeclampsia patients on preeclampsia progression was the focus of this investigation.
The aim of the current study is to check if the levels of expression of Colon cancer-associated transcription factor 1 (CCAT1) and Cyclin-dependent protein kinase 4 (CDK4) are associated with preeclampsia vulnerability and biogenesis.
and methods: This work included the participation of 160 people. Eighty of the patients had preeclampsia. The control group included 80 normal pregnant women. The two groups were almost of the same age. A thorough clinical examination was performed in all groups (including taking a detailed history, concentrating on parity, age and previous background of diabetes or hypertension). The expression levels of CCAT1 and CDK4 in placental tissue were determined using a real-time q PCR technique.
Expression levels of CCAT1 and CDK4 differed significantly between the study groups. preeclamptic patients having the highest level of CCAT1in comparison with control group, However, preeclamptic patients having lower level of CDK4 than controls. There was a strong negative association between CDK4 expression level and DBP, SBP, creatinine, urea and CCAT1 level of expression in the preeclamptic group, whereas there was a positive correlation between CCAT1 level of expression and DBP, SBP, urea and creatinine in patients group.
Based on the findings of this study, it is possible that CCAT1 and CDK4 expression levels could be used to aid in the diagnosis and biogenesis of preeclampsia.
孕产妇和围产期死亡率由多种因素引起，包括先兆子痫。先兆子痫的发病和进展可能受长链非编码RNA（lncRNA）影响。本研究重点关注长链非编码RNA（lncRNA）结肠癌相关转录因子1（CCAT1）在先兆子痫患者胎盘中的表达对先兆子痫进展的影响。
本研究旨在检验结肠癌相关转录因子1（CCAT1）和细胞周期蛋白依赖性蛋白激酶4（CDK4）的表达水平是否与先兆子痫的易感性和发生有关。
本研究纳入160人。其中80例为先兆子痫患者。对照组包括80名正常孕妇。两组年龄相近。对所有组进行了全面的临床检查（包括详细询问病史，重点关注产次、年龄以及既往糖尿病或高血压病史）。采用实时定量PCR技术测定胎盘组织中CCAT1和CDK4的表达水平。
研究组之间CCAT1和CDK4的表达水平存在显著差异。与对照组相比，先兆子痫患者的CCAT1水平最高，然而，先兆子痫患者的CDK4水平低于对照组。在先兆子痫组中，CDK4表达水平与舒张压、收缩压（SBP）、肌酐、尿素和CCAT1表达水平呈强负相关，而在患者组中，CCAT1表达水平与舒张压、收缩压、尿素和肌酐呈正相关。
基于本研究结果，CCAT1和CDK4的表达水平有可能用于辅助先兆子痫的诊断和发病机制研究。
