Ramanaiah Illuri, Sudeep Heggar Venkataramana, Shyamprasad Kodimule
Department of Preclinical Studies, R&D Center for Excellence, Vidya Herbs Pvt Ltd, Bangalore, Karnataka, 560 105, India.
Diabetes Metab Syndr Obes. 2022 Jun 15;15:1819-1829. doi: 10.2147/DMSO.S366609. eCollection 2022.
Research on plant-based formulations has drawn considerable attention in the management of diabetic neuropathy (DN) for having lesser side effects than the synthetic counterparts. Here, we have investigated for the first time the therapeutic effects of a standardized L., (black pepper) seed extract, Viphyllin in mitigating hyperglycemia and neuropathic pain of type 2 diabetes model rats.
Type 2 diabetes was induced in male Wistar rats using high fat diet and a single dose of streptozotocin (60 mg/kg .). The diabetic rats were orally administered with Viphyllin containing not less than 30% β-caryophyllene (BCP), at 25 mg, 50 mg and 100 mg/kg/day doses for 6 weeks. Changes in body weight, fasting blood glucose (FBG), glucose tolerance, and blood biochemical parameters were measured. The nociceptive response to thermal stimulus (tail flick test) and sciatic nerve conduction velocity (NCV) were recorded at the end of study.
Viphyllin treatment markedly improved the body weight and glucose tolerance in diabetic rats. Also, the extract could significantly reduce the diabetes-induced elevation in FBG, liver and kidney indices. Further, Viphyllin dose-dependently increased the nociception latency in tail flick test compared to untreated diabetic rats (<0.05). Viphyllin at 100 mg/kg significantly increased the NCV (44.12±1.91*** m/s vs diabetic control 25.80±1.88 m/s). The antioxidant enzyme activities in sciatic nerve tissue were considerably increased in Viphyllin-treated groups compared to diabetic control. A 6-week treatment with Viphyllin markedly reversed the pathological manifestations of diabetes in vital organs such as liver, kidney and pancreas.
The study concludes that Viphyllin exerts antidiabetic effects and improves nerve conduction to mitigate neuropathic pain.
基于植物的制剂研究在糖尿病神经病变（DN）的管理中引起了相当大的关注，因为其副作用比合成制剂少。在此，我们首次研究了标准化的黑胡椒种子提取物Viphyllin对减轻2型糖尿病模型大鼠高血糖和神经性疼痛的治疗效果。
使用高脂肪饮食和单剂量链脲佐菌素（60mg/kg）诱导雄性Wistar大鼠患2型糖尿病。给糖尿病大鼠口服含不少于30%β-石竹烯（BCP）的Viphyllin，剂量分别为25mg、50mg和100mg/kg/天，持续6周。测量体重、空腹血糖（FBG）、葡萄糖耐量和血液生化参数的变化。在研究结束时记录对热刺激的伤害性反应（甩尾试验）和坐骨神经传导速度（NCV）。
Viphyllin治疗显著改善了糖尿病大鼠的体重和葡萄糖耐量。此外，该提取物可显著降低糖尿病引起的FBG、肝脏和肾脏指标升高。此外，与未治疗的糖尿病大鼠相比，Viphyllin在甩尾试验中剂量依赖性地增加了伤害感受潜伏期（<0.05）。100mg/kg的Viphyllin显著增加了NCV（44.12±1.91***m/s，而糖尿病对照组为25.80±1.88m/s）。与糖尿病对照组相比，Viphyllin治疗组坐骨神经组织中的抗氧化酶活性显著增加。Viphyllin为期6周的治疗显著逆转了肝脏、肾脏和胰腺等重要器官中糖尿病的病理表现。
该研究得出结论，Viphyllin具有抗糖尿病作用，并改善神经传导以减轻神经性疼痛。
