Li Dan, Zhao Linlu, Qian Jin, Liu Heng, You Jinmao, Cheng Ziyi, Yu Fabiao
Key Laboratory of Life-Organic Analysis of Shandong Province, School of Chemistry and Chemical Engineering, Qufu Normal University Qufu 273165 PR China
Laboratory of Neurology, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University Haikou 571199 China
RSC Adv. 2022 May 26;12(25):15910-15917. doi: 10.1039/d2ra02813a. eCollection 2022 May 23.
Considering the pivotal role of biomarkers in plasma, the development of biomarker specific sensing platforms is of great significance to achieve accurate diagnosis and monitor the occurrence and progress in acute kidney injury (AKI). In this paper, we develop a promising surface-enhanced Raman scattering-based aptasensor for duplex detection of two protein biomarkers in AKI. Exploiting the base-pairing specificity of nucleic acids to form a Y-shaped self-assembled aptasensor, the MGITC labelled gold nanoparticles will be attached to the surface of magnetic beads. In the presence of specific AKI-related biomarkers, the gold nanoparticles will detach from magnetic beads into the supernatant, thus leading to a SERS signal increase, which can be used for the highly sensitive analysis of target biomarkers. In addition, the limit of detection calculated for each biomarker indicates that the SERS-based aptasensor can well meet the detection requirements in clinical applications. Finally, the generality of this sensor in the early diagnosis of AKI is confirmed by using a rat model and spiked plasma samples. This sensing platform provides a facile and general route for sensitive SERS detection of AKI-related biomarkers, which offers great promising utility for and accurate practical bioassay in AKI early diagnosis.
鉴于生物标志物在血浆中的关键作用，开发生物标志物特异性传感平台对于实现急性肾损伤（AKI）的准确诊断以及监测其发生和进展具有重要意义。在本文中，我们开发了一种基于表面增强拉曼散射的有前景的适体传感器，用于对AKI中的两种蛋白质生物标志物进行双检测。利用核酸的碱基配对特异性形成Y形自组装适体传感器，MGITC标记的金纳米颗粒将附着在磁珠表面。在存在特定的AKI相关生物标志物时，金纳米颗粒将从磁珠上脱离进入上清液，从而导致表面增强拉曼散射信号增加，这可用于对目标生物标志物进行高灵敏度分析。此外，为每种生物标志物计算的检测限表明，基于表面增强拉曼散射的适体传感器能够很好地满足临床应用中的检测要求。最后，通过使用大鼠模型和加标血浆样本，证实了该传感器在AKI早期诊断中的通用性。这种传感平台为灵敏地表面增强拉曼散射检测AKI相关生物标志物提供了一种简便通用的途径，这为AKI早期诊断中的准确实用生物测定提供了极具前景的效用。
