Wei Xue-Ping, Zhang Xiao-Yi, Dong Yu-Qing, Cheng Ji-Long, Bai Yun-Jun, Liu Jiu-Shi, Qi Yao-Dong, Zhang Ben-Gang, Liu Hai-Tao
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Engineering Research Center of Tradition Chinese Medicine Resource, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Front Plant Sci. 2022 Jun 6;13:878263. doi: 10.3389/fpls.2022.878263. eCollection 2022.
Most plants of have an important medicinal and economic value with a long history, and ("Gaoben") has long been used in traditional Chinese medicine for the treatment of carminative, dispelling cold, dehumidification, and analgesia. While in the market (Xinjiang Gaoben) is substitution for Gaoben, and occupies a higher market share. These three Gaoben-related medicinal materials are similar in morphology, and are difficult to distinguish from each other by the commonly used DNA barcodes. The chloroplast genome has been widely used for molecular markers, evolutionary biology, and barcoding identification. In this study, the complete chloroplast genome sequences of , , and were reported. The results showed that the complete chloroplast genomes of these three species have typical quadripartite structures, which were comprised of 148,664, 148,539, and 148,497 bp. A total of 114 genes were identified, including 81 protein-coding genes (PCGs), 29 tRNA genes, and four rRNA genes. Our study indicated that highly variable region 2-L and D-4 that can be used as specific DNA barcodes to distinguish and identify , , and . In addition, phylogenetic study showed that nested in and as a sister group of and , which suggested these two genera are both in need of revision. This study offer valuable information for future research in the identification of Gaoben-related medicinal materials and will benefit for further phylogenetic study of Apiaceae.
大多数植物都具有重要的药用和经济价值，历史悠久，而藁本长期以来一直被用于传统中药中，用于理气、散寒、除湿和止痛。而在市场上，新疆藁本替代了藁本，并占据了更高的市场份额。这三种与藁本相关的药材在形态上相似，难以通过常用的DNA条形码相互区分。叶绿体基因组已被广泛用于分子标记、进化生物学和条形码鉴定。在本研究中，报道了藁本、新疆藁本和辽藁本的完整叶绿体基因组序列。结果表明，这三个物种的完整叶绿体基因组具有典型的四分体结构，分别由148,664、148,539和148,497 bp组成。共鉴定出114个基因，包括81个蛋白质编码基因（PCG）、29个tRNA基因和4个rRNA基因。我们的研究表明，高变区2-L和D-4可作为特异性DNA条形码用于区分和鉴定藁本、新疆藁本和辽藁本。此外，系统发育研究表明，新疆藁本嵌套在藁本中，并作为辽藁本的姐妹群，这表明这两个属都需要修订。本研究为今后藁本相关药材的鉴定研究提供了有价值的信息，并将有助于伞形科的进一步系统发育研究。
