细菌膜脂组成中甲基-(Z)-11-十四碳烯酸在抗生素耐药性中的作用。

The Role of Methyl-(Z)-11-tetradecenoate Acid from the Bacterial Membrane Lipid Composition in Antibiotic Resistance.

机构信息

Banat's University of Agricultural Sciences and Veterinary Medicine Timisoara, Calea Aradului 119, 300645 Timisoara, Romania.

University of Medicine and Pharmacy Timisoara, Piata Eftimie Murgu, 2, 300041 Timisoara, Romania.

出版信息

Biomed Res Int. 2022 Jun 13;2022:6028045. doi: 10.1155/2022/6028045. eCollection 2022.

DOI:10.1155/2022/6028045

PMID:35734346

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9209004/

Abstract

BACKGROUND

The bacterial membrane plays a critical role in the survival of bacteria and the effectiveness of antimicrobial peptides in protecting the host. The lipid constituents of the bacterial membrane are not evenly distributed, and they could be affected by clustering anionic lipids with cationic peptides with multiple positive charges. That could be harmful to bacteria because it prevents lipids from interacting with other molecular components of the cell membrane, disrupts existing natural domains, or creates phase boundary defects between the clustered lipids and the bulk of the membrane. This preliminary quantitative study is aimed at assembling a correlation between antibiotic resistance and bacterial lipid composition in , based on the function and arrangement of the bilipid coating of the bacterial cell, intimately associated with the path of antimicrobials through membranes.

METHODS

Fifteen multiresistant samples are collected from swine with enterocolitis tested for resistance levels using the disc diffusimetric method (Kirby-Bauer disc diffusion). Pathogen identification completed using the API 20E multitest system revealed the presence in 11 samples. In these samples, bacterial membrane detection of fatty acid methyl esters (FAME) operating a 240 MS Ion Trap (Varian) GC/MS (Agilent Technologies, Santa Clara, CA, USA) was performed, using the MIDI Sherlock recognition software model.

RESULTS

Interpreting the descriptive statistical method, the correlation matrix, and regression curves and after ANOVA analysis, we ascertained that the studied population statistically confirmed different degrees of resistance in most of the samples analyzed in this test.

CONCLUSIONS

In one case, the methyl-(Z)-11-tetradecenoate acid was observed to have a relationship with the susceptibility evaluation by using the disc diffusimetric method, which has revealed the lowest rate of antimicrobial resistance, so it has importance in further resistance evaluation studies.

摘要

背景

细菌膜在细菌的生存和抗菌肽保护宿主方面起着至关重要的作用。细菌膜的脂质成分分布不均匀，它们可能会受到带多个正电荷的阳离子肽与阴离子脂质聚集的影响。这对细菌是有害的，因为它阻止了脂质与细胞膜其他分子成分的相互作用，破坏了现有的天然结构域，或者在聚集的脂质和膜的大部分之间产生了相界缺陷。这项初步的定量研究旨在根据细菌细胞双层脂质涂层的功能和排列，建立一种与抗生素通过细胞膜的途径密切相关的细菌脂质组成与抗生素耐药性之间的相关性。

方法

从患有肠炎的猪身上采集了 15 个多耐药样本，使用纸片扩散法（Kirby-Bauer 纸片扩散法）检测耐药水平。使用 API 20E 多测试系统完成病原体鉴定，在 11 个样本中发现了的存在。在这些样本中，使用 240 MS Ion Trap（Varian）GC/MS（Agilent Technologies，Santa Clara，CA，USA）对脂肪酸甲酯（FAME）进行细菌膜检测，使用 MIDI Sherlock 识别软件模型。