State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing, China.
Institute for Acute Communicable Disease Prevention and Control, Yunnan Center for Disease Control and Prevention, Kunming, Yunnan, China.
Gut Microbes. 2022 Jan-Dec;14(1):2089007. doi: 10.1080/19490976.2022.2089007.
Some serovars of are not or rare found to cause salmonellosis in human. In our clinic-based surveillance, three rare 4,5,12:a:- strains were recovered from three patients with diarrhea. To explore their genetic and epidemiological characteristics and pathogenesis, we conducted whole-genome sequencing, invasion assays in mammalian cells, and virulence assays in an animal model. The three isolates had indistinguishable molecular patterns and similar genome sequences, and clustered together with an isolate from edible fish traded among countries. The isolates had biochemical reactions identical with those of subspecies but belonged to subspecies according to genome phylogeny, revealing a new serovar, subsp. II serovar 4,5,12:a:-. The strains contained multiple virulence genes, elicited temporary bacteremia and enteritidis and caused cell damage in the mouse liver and cecum. This study provides evidence that this new serovar can infect humans and cause clusters of cases, and whole-genome sequencing detection and surveillance of can help to accurately define classification and clonality, improve diagnosis, facilitate outbreak detection and aid in the source tracing of salmonellosis epidemics.
有些 血清型不易或很少引起人类沙门氏菌病。在我们的基于临床的监测中，从三名腹泻患者中分离到三株罕见的 4,5,12:a:- 血清型 。为了探讨其遗传、流行病学特征和发病机制，我们进行了全基因组测序、哺乳动物细胞侵袭试验和动物模型中的毒力试验。这三个分离株具有相同的分子模式和相似的基因组序列，与来自国家间交易的食用鱼的分离株聚集在一起。这些分离株的生化反应与亚种完全相同，但根据基因组系统发育属于亚种，揭示了一个新的血清型，即 亚 II 血清型 4,5,12:a:-。这些菌株含有多个毒力基因，可引起暂时的菌血症和肠炎，并导致小鼠肝脏和盲肠细胞损伤。本研究提供了证据表明，这种新的血清型可以感染人类并引起群集性病例，全基因组测序检测和监测 可以帮助准确定义沙门氏菌的分类和克隆性，提高诊断水平，有助于发现暴发，并有助于追踪沙门氏菌病流行的源头。
