Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, China.
Environ Toxicol Chem. 2022 Sep;41(9):2305-2317. doi: 10.1002/etc.5415. Epub 2022 Aug 9.
While zebrafish (Danio rerio) have been accepted worldwide for evaluating chemical hazards to aquatic vertebrates, and in some countries it is mandated to generate fish toxicity data using native species, such as Chinese rare minnow (Gobiocypris rarus) in China. This represents an additional regulatory constraint that may cause redundant tests, additional animal uses, and higher costs. Previous studies showed that juvenile G. rarus was more sensitive than zebrafish juveniles and embryos to metals. To better understand the sensitivity of G. rarus to organic chemicals, we selected 29 fragrance ingredients belonging to various chemical classes and with differing physicochemical properties, for which good quality zebrafish acute toxicity data were available and tested them with juvenile G. rarus and embryo D. rerio using the Organisation of Economic Co-operation and Development test guidelines. Chemical toxicity distribution (CTD) and chemical ratio distribution (CRD) models were established to systematically compare the sensitivity between juveniles of G. rarus and D. rerio, as well as between D. rerio embryos and juveniles. The results of the CTD models showed that for tested chemicals, the sensitivity of juvenile G. rarus was similar to that of D. rerio juveniles and embryos. The CRD comparisons revealed that juvenile G. rarus was slightly less sensitive by a factor of ~2 than juvenile D. rerio to ingredients belonging to Verhaar class 3 and Ecological Structure Activity Relationship ester class, while comparable to other chemicals. These comparative experiments demonstrated that fish toxicity data with G. rarus can be submitted for use in chemical registrations outside China, which would avoid repeating animal tests using D. rerio. Meanwhile, the similar sensitivity of zebrafish juveniles and embryos to fragrance ingredients confirmed the suitability of replacing juveniles by zebrafish embryos. Environ Toxicol Chem 2022;41:2305-2317. © 2022 SETAC.
虽然斑马鱼（Danio rerio）已被全球接受用于评估水生脊椎动物的化学危害，并且在一些国家，必须使用本土物种（如中国稀有鮈鲫（Gobiocypris rarus））来生成鱼类毒性数据，但这代表了额外的监管限制，可能导致重复测试、增加动物使用和提高成本。先前的研究表明，幼年中国稀有鮈鲫比斑马鱼幼鱼和胚胎对金属更敏感。为了更好地了解中国稀有鮈鲫对有机化学品的敏感性，我们选择了 29 种属于各种化学类别的香精成分，这些成分具有不同的物理化学性质，并且有高质量的斑马鱼急性毒性数据可用，并用这些成分测试了幼年中国稀有鮈鲫和胚胎斑马鱼，使用的是经济合作与发展组织的测试指南。建立了化学毒性分布（CTD）和化学比值分布（CRD）模型，以系统比较中国稀有鮈鲫幼鱼和斑马鱼幼鱼以及斑马鱼胚胎之间的敏感性。CTD 模型的结果表明，对于测试的化学物质，中国稀有鮈鲫幼鱼的敏感性与斑马鱼幼鱼和胚胎相似。CRD 比较表明，与属于 Verhaar 第 3 类和生态结构活性关系酯类的成分相比，中国稀有鮈鲫幼鱼对成分的敏感性比斑马鱼幼鱼低约 2 倍，而与其他化学物质相当。这些比较实验表明，使用中国稀有鮈鲫获得的鱼类毒性数据可用于中国以外的化学品注册，从而避免使用斑马鱼重复动物测试。同时，斑马鱼幼鱼和胚胎对香精成分的敏感性相似，证实了用斑马鱼胚胎替代幼鱼的适宜性。环境毒理化学 2022；41：2305-2317。© 2022 SETAC。
