免疫酶组织化学法检测弥散性血管内凝血大鼠的纤维蛋白微血栓
Immunoenzymehistochemical detection of fibrin microthrombi during disseminated intravascular coagulation rats.
作者信息
Craane H, Emeis J J, Lindeman J, Nieuwenhuizen W
出版信息
Histochemistry. 1978 Aug 25;57(2):97-105. doi: 10.1007/BF00496674.
In tissue of rats with disseminated intravascular coagulation, fibrin microthrombi can be sensitively detected by immunohistochemical methods, using antisera against rat fibrinogen or fibrin monomer. An indirect immunoperoxidase procedure on paraplast-embedded sections yields best results with regard to the morphology of the thrombi and their localization in the tissue. Only fibrillar immunoreactive material, oriented lengthwise in the vessels, should be regarded as microthrombi formed in vivo.
在患有弥散性血管内凝血的大鼠组织中，使用抗大鼠纤维蛋白原或纤维蛋白单体的抗血清，通过免疫组织化学方法可灵敏地检测到纤维蛋白微血栓。对于包埋在石蜡中的切片，采用间接免疫过氧化物酶法，在血栓的形态及其在组织中的定位方面可获得最佳结果。只有在血管中沿长度方向排列的纤维状免疫反应性物质才应被视为体内形成的微血栓。
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