Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China.
Biosensors (Basel). 2022 Jun 13;12(6):406. doi: 10.3390/bios12060406.
Alkaline phosphatase (ALP) is a significant biomarker that indicates osteoblast activity and skeletal growth. Efficient ALP detection methods are essential in drug development and clinical diagnosis. In this work, we developed an in-situ synthesized three-dimensional graphene networks (3DGNs)-based electrochemical sensor to determine ALP activity. The sensor employs an ALP enzymatic conversion of non-electroactive substrate to electroactive product and presents the ALP activity as an electrochemical signal. With 3DGNs as the catalyst and signal amplifier, a sample consumption of 5 μL and an incubation time of 2 min are enough for the sensor to detect a wide ALP activity range from 10 to 10,000 U/L, with a limit of detection of 5.70 U/L. This facile fabricated sensor provides a quick response, cost-effective and non-destructive approach for monitoring living adherent osteoblast cell activity and holds promise for ALP quantification in other biological systems and clinical samples.
碱性磷酸酶（ALP）是一种重要的生物标志物，可指示成骨细胞活性和骨骼生长。高效的 ALP 检测方法在药物开发和临床诊断中至关重要。在这项工作中，我们开发了一种基于原位合成的三维石墨烯网络（3DGNs）的电化学传感器，用于测定 ALP 活性。该传感器利用 ALP 酶促将非电活性底物转化为电活性产物，并将 ALP 活性作为电化学信号呈现。由于 3DGNs 可用作催化剂和信号放大器，因此传感器仅需 5 μL 的样品消耗和 2 分钟的孵育时间，即可检测出 10 至 10,000 U/L 的宽 ALP 活性范围，检测限低至 5.70 U/L。这种易于制造的传感器提供了一种快速响应、经济高效且非破坏性的方法，可用于监测活贴壁成骨细胞的活性，有望用于其他生物系统和临床样本中的 ALP 定量。
