Department of Chemical Engineering, Kwangwoon University, Seoul 01897, Korea.
Department of Convergence Medical Sciences, School of Medicine, Pusan National University, Yangsan 50612, Korea.
Biosensors (Basel). 2022 Jun 15;12(6):418. doi: 10.3390/bios12060418.
Since 2010, DNA nanotechnology has advanced rapidly, helping overcome limitations in the use of DNA solely as genetic material. DNA nanotechnology has thus helped develop a new method for the construction of biosensors. Among bioprobe materials for biosensors, nucleic acids have shown several advantages. First, it has a complementary sequence for hybridizing the target gene. Second, DNA has various functionalities, such as DNAzymes, DNA junctions or aptamers, because of its unique folded structures with specific sequences. Third, functional groups, such as thiols, amines, or other fluorophores, can easily be introduced into DNA at the 5' or 3' end. Finally, DNA can easily be tailored by making junctions or origami structures; these unique structures extend the DNA arm and create a multi-functional bioprobe. Meanwhile, nanomaterials have also been used to advance plasmonic biosensor technologies. Nanomaterials provide various biosensing platforms with high sensitivity and selectivity. Several plasmonic biosensor types have been fabricated, such as surface plasmons, and Raman-based or metal-enhanced biosensors. Introducing DNA nanotechnology to plasmonic biosensors has brought in sight new horizons in the fields of biosensors and nanobiotechnology. This review discusses the recent progress of DNA nanotechnology-based plasmonic biosensors.
自 2010 年以来，DNA 纳米技术发展迅速，有助于克服 DNA 仅作为遗传物质的使用限制。因此，DNA 纳米技术帮助开发了一种构建生物传感器的新方法。在生物传感器的生物探针材料中，核酸具有几个优势。首先，它具有与靶基因杂交的互补序列。其次，由于其具有特定序列的独特折叠结构，DNA 具有各种功能，例如 DNA 酶、DNA 连接或适体。第三，巯基、胺或其他荧光团等功能基团可以很容易地在 5' 或 3' 端引入 DNA。最后，通过形成连接或折纸结构可以轻松地对 DNA 进行定制；这些独特的结构延伸了 DNA 臂并创建了多功能生物探针。同时，纳米材料也被用于推进等离子体生物传感器技术。纳米材料为各种具有高灵敏度和选择性的生物传感平台提供了支持。已经制造了几种等离子体生物传感器类型，例如表面等离子体、基于拉曼或金属增强的生物传感器。将 DNA 纳米技术引入等离子体生物传感器为生物传感器和纳米生物技术领域带来了新的视野。本文综述了基于 DNA 纳米技术的等离子体生物传感器的最新进展。
