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松口蘑生境中的真菌-细菌网络及其分布规律的驱动因素

Fungal-Bacterial Networks in the Habitat of SongRong () and Driving Factors of Their Distribution Rules.

作者信息

Ji Rui-Qing, Xu Yang, Si Yan-Ji, Phukhamsakda Chayanard, Li Yu, Meng Li-Peng, Liu Shu-Yan, Xie Meng-Le

机构信息

Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

Jilin Forestry Science Institute, Changchun 130032, China.

出版信息

J Fungi (Basel). 2022 May 27;8(6):575. doi: 10.3390/jof8060575.

Abstract

Soil origin, mycorrhizal plant partners and environmental factors affect the growth and development of SongRong (). In order to clarify the relationships of fungi-bacteria networks and various influence factors in the habitat of SongRong, we chose three collection sites with a pure forest (plot A without SongRong was used as the control sample site), mixed (plot B) and mixed with and (plot C). By using high-throughput sequencing, we obtained a total of 4930 fungal and 55501 bacterial amplicon sequence variants (ASVs) based on internally transcribed spacer ribosomal RNA (ITS rRNA) and 16S ribosomal RNA (16S rRNA) sequencing via the Illumina NovaSeq platform. In the habitat soil of SongRong (plot B and plot C), alpha or beta diversity and species compositions of fungi and bacteria were different from plot A. The fungal-bacterial networks follow the selection rule that few dominant genera account for the greater relative abundance. Forest types, but not the host itself, drove the fungal-bacterial networks of the forest soil, and soil physicochemical characteristics and texture affected their abundance. The abundance of was affected by the fungal and bacterial abundance in the habitat.

摘要

土壤来源、菌根植物伙伴和环境因素会影响松茸的生长发育。为了阐明松茸栖息地中真菌-细菌网络与各种影响因素之间的关系,我们选择了三个采集地点,一个是纯林(将没有松茸的A地块用作对照样本地点),一个是混交林(B地块),还有一个是与[具体内容缺失]混交的林(C地块)。通过高通量测序,我们基于内部转录间隔区核糖体RNA(ITS rRNA)和16S核糖体RNA(16S rRNA)测序,借助Illumina NovaSeq平台,总共获得了4930个真菌和55501个细菌扩增子序列变体(ASV)。在松茸的栖息地土壤(B地块和C地块)中,真菌和细菌的α或β多样性以及物种组成与A地块不同。真菌-细菌网络遵循少数优势属占相对丰度较大比例的选择规则。森林类型而非宿主本身驱动了森林土壤的真菌-细菌网络,土壤理化特性和质地影响它们的丰度。[具体内容缺失]的丰度受到栖息地中真菌和细菌丰度的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a84/9225054/d4285c6e2a98/jof-08-00575-g001.jpg

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