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菌根圈中真菌的多样菌根形态、群落结构及动态

The Diverse Mycorrizal Morphology of , the Fungal Communities Structure and Dynamics from the Mycorrhizosphere.

作者信息

Liu Jin, Xu Yang, Si Yan-Ji, Li Bin-Qi, Chen Peng, Wu Ling-Ling, Guo Pu, Ji Rui-Qing

机构信息

Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.

出版信息

J Fungi (Basel). 2024 Jan 14;10(1):65. doi: 10.3390/jof10010065.

Abstract

It is generally believed that mycorrhiza is a microecosystem composed of mycorrhizal fungi, host plants and other microscopic organisms. The mycorrhiza of is more complex and the diverse morphology of our investigated results displays both typical ericoid mycorrhizal characteristics and ectomycorrhizal traits. The characteristics of ectendoomycorrhiza, where mycelial invade from the outside into the root cells, have also been observed. In order to further clarify the mycorrhizal fungi members and other fungal communities of mycorrhiza, and explore the effects of vegetation and soil biological factors on their community structure, we selected two woodlands in the northeast of China as samples-one is a mixed forest of and , and the other a mixed forest of , , and . The sampling time was during the local growing season, from June to September. High-throughput sequencing yielded a total of 3020 fungal amplicon sequence variants (ASVs), which were based on sequencing of the internal transcribed spacer ribosomal RNA (ITS rRNA) via the Illumina NovaSeq platform. In the different habitats of , there are differences in the diversity of fungi obtained from mycorrhizal niches, and specifically the mycorrhizal fungal community structure in the complex vegetation of mixed forests, where is found, exhibits greater stability, with relatively minor changes over time. Soil fungi are identified as the primary source of fungi within the mycorrhizal niche, and the abundance of mycorrhizal fungi from mycorrhizal niches in is significantly influenced by soil pH, organic matter, and available nitrogen. The relationship between soil fungi and mycorrhizal fungi from mycorrhizal niches is simultaneously found to be intricate, while the genus emerges as a central genus among mycorrhizal fungi from mycorrhizal niches. However, there is currently a substantial gap in the foundational research of this genus, including the fact that mycorrhizal fungi from mycorrhizal niches have, compared to fungi present in the soil, proven to be more sensitive to changes in soil moisture.

摘要

一般认为,菌根是由菌根真菌、寄主植物和其他微生物组成的微生态系统。[具体植物名称]的菌根更为复杂,我们的研究结果显示出多样的形态,兼具典型的石楠类菌根特征和外生菌根特征。还观察到内外生菌根的特征,即菌丝从外部侵入根细胞。为了进一步阐明[具体植物名称]菌根的菌根真菌成员和其他真菌群落,并探讨植被和土壤生物因素对其群落结构的影响,我们选择了中国东北的两片林地作为样本——一片是[具体植物名称1]和[具体植物名称2]的混交林,另一片是[具体植物名称3]、[具体植物名称4]和[具体植物名称5]的混交林。采样时间为当地生长季节,即6月至9月。高通量测序通过Illumina NovaSeq平台对核糖体RNA的内部转录间隔区(ITS rRNA)进行测序,共产生了3020个真菌扩增子序列变体(ASV)。在[具体植物名称]的不同生境中,从菌根生态位获得的真菌多样性存在差异,特别是在发现[具体植物名称]的混交林复杂植被中的菌根真菌群落结构表现出更大的稳定性,随时间变化相对较小。土壤真菌被确定为菌根生态位内真菌的主要来源,[具体植物名称]菌根生态位中菌根真菌的丰度受土壤pH值、有机质和有效氮的显著影响。同时发现土壤真菌与菌根生态位中的菌根真菌之间的关系错综复杂,而[具体属名]属在菌根生态位的菌根真菌中成为核心属。然而,目前该属的基础研究存在很大差距,包括与土壤中存在的真菌相比,菌根生态位中的菌根真菌对土壤水分变化更为敏感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b18/10817234/9b03c4ae4aae/jof-10-00065-g001.jpg

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