Wang Ping, Xu Shuang, Tang Yuqi, Wang Hong, Bai Xuelian, Zhang Huawei
School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
J Fungi (Basel). 2022 May 31;8(6):591. doi: 10.3390/jof8060591.
is one of the most important sources of secondary metabolites (SMs), with a wide array of pharmacological effects, including anti-inflammatory, antitumor, immunomodulatory and antioxidant effects. However, the biosynthetic analysis of these bioactive components has been rarely reported owing to the lack of high-quality genome sequences and comprehensive analysis. In this study, the whole genome of one marine-sponge-derived strain L14 was sequenced and assembled as well as in-depth bioinformatic analysis. The results indicated that the sequence assembly of strain L14 generated one high-quality genome with a total size of 36.1 Mb, a G + C content of 45.3% and an N50 scaffold of 4.2 Mb. Gene annotation was extensively deployed using various BLAST databases, including non-redudant (Nr) protein sequence, nucleotide (Nt) sequence, Swiss-Prot, Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) as well as Pathogen Host Interactions (PHI) and Carbohydrate-active enzymes (CAZy) databases. AntiSMASH analysis revealed that this marine strain harbors a total of 69 SMs biosynthesis gene clusters (BGCs), including 17 PKSs, 18 NRPSs, 21 NRPS-likes, 9 terpenes, 2 indoles, 1 betalactone and 1 siderophore, suggesting its biosynthetic potential to produce a wide variety of SMs. These findings will assist in future investigations on the genetic basis of strain L14 and provide insights into its new bioactive SMs for new drug discovery.
是次生代谢产物（SMs）最重要的来源之一，具有广泛的药理作用，包括抗炎、抗肿瘤、免疫调节和抗氧化作用。然而，由于缺乏高质量的基因组序列和全面的分析，这些生物活性成分的生物合成分析鲜有报道。在本研究中，对一株海洋海绵来源的菌株L14进行了全基因组测序、组装以及深入的生物信息学分析。结果表明，菌株L14的序列组装产生了一个高质量的基因组，总大小为36.1 Mb，G + C含量为45.3%，N50支架为4.2 Mb。使用各种BLAST数据库进行了广泛的基因注释，包括非冗余（Nr）蛋白质序列、核苷酸（Nt）序列、Swiss-Prot、基因本体（GO）、京都基因与基因组百科全书（KEGG）、直系同源簇（COG）以及病原体宿主相互作用（PHI）和碳水化合物活性酶（CAZy）数据库。抗SMASH分析表明，该海洋菌株总共含有69个SMs生物合成基因簇（BGCs），包括17个聚酮合酶（PKSs）、18个非核糖体肽合成酶（NRPSs）、21个类NRPSs、9个萜类、2个吲哚、1个β-内酰胺和1个铁载体，表明其具有产生多种SMs的生物合成潜力。这些发现将有助于未来对菌株L14遗传基础的研究，并为发现新的生物活性SMs用于新药研发提供见解。
