Alghuthaymi Mousa A, Danaraj Jeyapragash, Albarakaty Fawziah M, Periakaruppan Rajiv, Vajravelu Manigandan, Ayyappan Saravanakumar, Selvaraj Kumaralingam, Abd-Elsalam Kamel A
Biology Department, Science and Humanities College, Shaqra University, Alquwayiyah 11726, Saudi Arabia.
Centre for Ocean Research (DST-FIST Sponsored Centre), MoES-Earth Science and Technology Cell (Marine Biotechnological Studies), Col. Dr. Jeppiaar Research Park, Sathyabama Institute of Science and Technology, Chennai 600119, India.
J Fungi (Basel). 2022 Jun 7;8(6):609. doi: 10.3390/jof8060609.
Elicitors are the agents that stimulate the defense responses of plants, and accumulate specialized metabolites in plant tissue culture. This study investigated the elicitor-feeding response of suspension cell cultures (SCC) for metabolomics analysis and screening of specialized compounds against . Methyl jasmonate (MeJA) was used as an elicitor, and treatment of SCC at a concentration of 20 µM MeJA resulted in the maximum rosmarinic acid (RA) accumulation (117 mg/g dry weight), with transcript levels of RA biosynthetic genes HpPAL, HpC4H, and Hp4CL being 4.2, 2.5, and 3.7-fold higher, respectively, than the controls. GC-MS-based metabolomics analysis revealed a total of 47 metabolites, including 30 organic acids, six amino acids, six flavonoids, two sugars, two plant growth regulators, and one vitamin, which were significantly different between control and MeJA-treated cells. Furthermore, five phenolic acids were discovered at higher concentrations, including -anisic acid, -coumaric acid, caffeic acid, vanillic acid, and rosmarinic acid, and were purified and structurally elucidated for alternative antifungal screening against and the evaluation of ADMET properties. The results from antifungal screening revealed that RA at MIC of 31.25 mg/L exhibited the lowest growth percentage of (1.99%), with higher inhibition of isocitrate lyase 1 (ICL 1) enzyme (93.1%), followed by -anisic acid (86.2%) and caffeic acid (85.1%), respectively. The drug likeliness and ADMET properties of RA exhibited promising results, with a bioactivity score of 0.57, 0.15, and 0.24 for nuclear receptor ligand, protease inhibitor, and enzyme inhibitor, respectively. Therefore, MeJA appears to have a significant effect on enhanced RA accumulation in cells with phenylpropanoid transcript expression, and acts as an ICL1 inhibitor of .
激发子是刺激植物防御反应并在植物组织培养中积累特殊代谢产物的因子。本研究调查了悬浮细胞培养物（SCC）对激发子的反应，用于代谢组学分析和筛选针对……的特殊化合物。茉莉酸甲酯（MeJA）用作激发子，以20 µM MeJA的浓度处理SCC导致迷迭香酸（RA）积累量最大（117 mg/g干重），RA生物合成基因HpPAL、HpC4H和Hp4CL的转录水平分别比对照高4.2倍、2.5倍和3.7倍。基于气相色谱-质谱联用的代谢组学分析共鉴定出47种代谢产物，包括30种有机酸、6种氨基酸、6种黄酮类化合物、2种糖类、2种植物质生长调节剂和1种维生素，对照细胞和经MeJA处理的细胞之间这些代谢产物存在显著差异。此外，发现了5种浓度较高的酚酸，包括对甲氧基苯甲酸、对香豆酸、咖啡酸、香草酸和迷迭香酸，并对其进行了纯化和结构解析，用于针对……的替代抗真菌筛选以及药物代谢动力学、药物效应动力学性质评估。抗真菌筛选结果显示，MIC为31.25 mg/L的RA对……的生长抑制率最低（1.99%），对异柠檬酸裂解酶1（ICL 1）的抑制作用较强（93.1%），其次是对甲氧基苯甲酸（86.2%）和咖啡酸（85.1%）。RA的药物相似性和药物代谢动力学、药物效应动力学性质显示出有前景的结果，作为核受体配体、蛋白酶抑制剂和酶抑制剂的生物活性评分分别为0.57、0.15和0.24。因此，MeJA似乎对增强……细胞中具有苯丙烷类转录表达的RA积累有显著影响，并作为……的ICL1抑制剂发挥作用。
