Yan Meng-Qiu, Su Xiao-Wei, Liu Yan-Fang, Tang Chuan-Hong, Tang Qing-Jiu, Zhou Shuai, Tan Yi, Liu Li-Ping, Zhang Jing-Song, Feng Jie
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, China.
J Fungi (Basel). 2022 Jun 9;8(6):615. doi: 10.3390/jof8060615.
The effects of oleic acid addition methods on the metabolic flux distribution of ganoderic acids R, S and T's biosynthesis from were investigated. The results showed that adding filter-sterilized oleic acid in the process of submerged fermentation and static culture is of benefit to the synthesis of ganoderic acids R, S and T. The metabolic fluxes were increased by 97.48%, 78.42% and 43.39%, respectively. The content of ganoderic acids R, S and T were 3.11 times, 5.19 times and 1.44 times higher, respectively, than they were in the control group, which was without additional oleic acid. Ganoderic acids R, S and T's synthesis pathways (GAP), tricarboxylic acid cycles (TCA), pentose phosphate pathways (PP) and glycolysis pathways (EMP) were all enhanced in the process. Therefore, additional oleic acid can strengthen the overall metabolic flux distribution of in a submerged fermentation-static culture and it can reduce the accumulation of the by-product mycosterol. This study has laid an important foundation for improving the production of triterpenes in the submerged fermentation of .
研究了油酸添加方式对灵芝酸R、S和T生物合成代谢通量分布的影响。结果表明，在深层发酵和静置培养过程中添加过滤除菌的油酸有利于灵芝酸R、S和T的合成。代谢通量分别提高了97.48%、78.42%和43.39%。灵芝酸R、S和T的含量分别比未添加油酸的对照组高3.11倍、5.19倍和1.44倍。在此过程中，灵芝酸R、S和T的合成途径（GAP）、三羧酸循环（TCA）、磷酸戊糖途径（PP）和糖酵解途径（EMP）均得到增强。因此，添加油酸可增强深层发酵-静置培养中灵芝的整体代谢通量分布，并可减少副产物麦角甾醇的积累。本研究为提高灵芝深层发酵中三萜类化合物的产量奠定了重要基础。
