Wu Chen-Gao, Tian Jia-Long, Liu Rui, Cao Peng-Fei, Zhang Tian-Jun, Ren Ang, Shi Liang, Zhao Ming-Wen
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
Appl Environ Microbiol. 2017 Sep 29;83(20). doi: 10.1128/AEM.01289-17. Print 2017 Oct 15.
Putrescine is an important polyamine that participates in a variety of stress responses. Ornithine decarboxylase (ODC) is a key enzyme that catalyzes the biosynthesis of putrescine. A homolog of the gene encoding ODC was cloned from In the -silenced strains, the transcript levels of the gene and the putrescine content were significantly decreased. The -silenced strains were more sensitive to oxidative stress. The content of ganoderic acid was increased by approximately 43 to 46% in the -silenced strains. The content of ganoderic acid could be recovered after the addition of exogenous putrescine. Additionally, the content of reactive oxygen species (ROS) was significantly increased by approximately 1.3-fold in the -silenced strains. The ROS content was significantly reduced after the addition of exogenous putrescine. The gene transcript levels and the activities of four major antioxidant enzymes were measured to further explore the effect of putrescine on the intracellular ROS levels. Further studies showed that the effect of the ODC-mediated production of putrescine on ROS might be a factor influencing the biosynthesis of ganoderic acid. Our study reports the role of putrescine in large basidiomycetes, providing a basis for future studies of the physiological functions of putrescine in microbes. It is well known that ODC and the ODC-mediated production of putrescine play an important role in resisting various environmental stresses, but there are few reports regarding the mechanisms underlying the effect of putrescine on secondary metabolism in microorganisms, particularly in fungi. is gradually becoming a model organism for studying environmental regulation and metabolism. In this study, a homolog of the gene encoding ODC was cloned in We found that the transcript level of the gene and the content of putrescine were significantly decreased in the -silenced strains. The content of ganoderic acid was significantly increased in the -silenced strains. Further studies showed that the effect of the ODC-mediated production of putrescine on ROS might be a factor influencing the biosynthesis of ganoderic acid. Our study reports the role of putrescine in large basidiomycetes, providing a basis for future studies of the physiological functions of putrescine in microbes.
腐胺是一种重要的多胺，参与多种应激反应。鸟氨酸脱羧酶（ODC）是催化腐胺生物合成的关键酶。从……中克隆了编码ODC的基因的一个同源物。在……沉默菌株中，该基因的转录水平和腐胺含量显著降低。……沉默菌株对氧化应激更敏感。在……沉默菌株中，灵芝酸含量增加了约43%至46%。添加外源腐胺后，灵芝酸含量可恢复。此外，在……沉默菌株中，活性氧（ROS）含量显著增加了约1.3倍。添加外源腐胺后，ROS含量显著降低。测定了该基因的转录水平和四种主要抗氧化酶的活性，以进一步探讨腐胺对细胞内ROS水平的影响。进一步研究表明，ODC介导的腐胺产生对ROS的影响可能是影响灵芝酸生物合成的一个因素。我们的研究报道了腐胺在大型担子菌中的作用，为今后研究腐胺在微生物中的生理功能提供了依据。众所周知，ODC和ODC介导的腐胺产生在抵抗各种环境应激中起重要作用，但关于腐胺对微生物，特别是真菌次级代谢影响的机制报道很少。……正逐渐成为研究环境调节和代谢的模式生物。在本研究中，从……中克隆了编码ODC的基因的一个同源物。我们发现，在……沉默菌株中，该基因的转录水平和腐胺含量显著降低。……沉默菌株中灵芝酸含量显著增加。进一步研究表明，ODC介导的腐胺产生对ROS的影响可能是影响灵芝酸生物合成的一个因素。我们的研究报道了腐胺在大型担子菌中的作用，为今后研究腐胺在微生物中的生理功能提供了依据。
