The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural Universitygrid.80510.3c, Chengdu, China.
Laboratory of Molecular and Cellular Parasitology, Healthy Longevity Translational Research Programme and Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Microbiol Spectr. 2022 Aug 31;10(4):e0226921. doi: 10.1128/spectrum.02269-21. Epub 2022 Jun 23.
is a common protistan parasite inhabiting the gastrointestinal tract of a wide range of hosts including humans and domestic and wild animals. Many studies have revealed the associations between and gut microbiome in humans. However, only a few studies have focused on the associations between and gut microbiome of animals, especially in forest musk deer (). We investigated the effects of the colonization on the intestinal bacterial community compositions using amplicon sequencing targeting the V4 variable region of the 16S rRNA. Two subtypes of (ST5 and ST10) and -free (control) were included in this study. We found that compared with the forest musk deer without , ST10-colonized forest musk deer had higher bacterial richness and diversity, while ST5-colonized forest musk deer showed a comparable bacterial diversity. Likewise, beta diversity revealed significant differences in bacterial community structure between ST10-colonized and -free forest musk deer. The proportion of were significantly enriched in ST10-colonized forest musk deer. Bacterial community structure between ST5-colonized and -free forest musk deer did not differ significantly. The present study explored the associations between and gut microbial community of forest musk deer for the first time, and revealed ST10 colonization, instead of ST5, is associated with higher bacterial diversity and shifted microbial structure. Our data provides valuable insights into the associations between gut microbiomes and parasites. Forest musk deer is listed as an endangered species by International Union for Conservation of Nature Red List, and the Chinese government has introduced captivity breeding measures to curb the rapid decline of the musk deer population since the 1950s. It has been suggested that colonization can modulate the composition of the host's intestinal microbiota, thereby affecting the host health. The present study investigated the effects of the colonization on the gut microbiota in the feces of forest musk deer in Sichuan Province, China. Two subtypes (ST5 and ST10) have differential effects on the bacterial diversity and community composition, suggesting that the study of should be distinguished at the subtype level. Because the pathogenicity of is controversial, pathogenic, or commensal, continuous monitoring of the impact of colonization on the intestinal microbiota is of great significance to assess its health effects on forest musk deer.
是一种常见的原生动物寄生虫，栖息在包括人类和家养及野生动物在内的广泛宿主的胃肠道中。许多研究揭示了人类中 和肠道微生物组之间的关联。然而，只有少数研究关注动物，特别是林麝（）中 和肠道微生物组之间的关联。我们使用针对 16S rRNA V4 可变区的扩增子测序来研究 定植对肠道细菌群落组成的影响。本研究包括两种 亚型（ST5 和 ST10）和无 （对照）。我们发现，与没有 的林麝相比，ST10 定植的林麝具有更高的细菌丰富度和多样性，而 ST5 定植的林麝表现出可比的细菌多样性。同样，β多样性揭示了 ST10 定植和无 的林麝之间细菌群落结构的显著差异。在 ST10 定植的林麝中， 的比例显著富集。ST5 定植和无 的林麝之间的细菌群落结构没有显著差异。本研究首次探索了林麝 和肠道微生物群落之间的关联，揭示了 ST10 定植，而不是 ST5，与更高的细菌多样性和微生物结构的改变有关。我们的数据为肠道微生物群和寄生虫之间的关联提供了有价值的见解。林麝被国际自然保护联盟濒危物种红色名录列为濒危物种，中国政府自 20 世纪 50 年代以来采取了圈养繁殖措施来遏制麝鹿数量的快速下降。有人建议， 定植可以调节宿主肠道微生物群的组成，从而影响宿主健康。本研究调查了 在中国四川省林麝粪便中的定植对肠道微生物群的影响。两种亚型（ST5 和 ST10）对细菌多样性和群落组成有不同的影响，这表明 研究应该在亚型水平上加以区分。由于 的致病性存在争议，是致病的还是共生的，因此持续监测 定植对肠道微生物群的影响对于评估其对林麝的健康影响具有重要意义。
