Mandrone Manuela, Marincich Lorenzo, Chiocchio Ilaria, Zannini Piero, Guarino Riccardo, Poli Ferruccio
Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio, 42, 40126 Bologna, Italy.
BIOME Lab, Department of Biological, Geological and Environmental Sciences, Alma Mater Studiorum-University of Bologna, Via Irnerio, 42, 40126 Bologna, Italy.
Metabolites. 2022 Jun 9;12(6):533. doi: 10.3390/metabo12060533.
The Aeolian Islands (Italy) are a volcanic archipelago in the Tyrrhenian Sea comprising seven main islands, among which are two active volcanoes. The peculiar geological features and the wide variety of environments and soils have an important impact on native plants, and in particular, the Aeolian populations of (a perennial cool-season bunchgrass) exhibit remarkable phenotypic variability. Considering that environmental drivers also strongly affect the production of plant metabolites, this work aimed at comparing the metabolomic profiles of (leaves) harvested at different altitudes on four islands of the Aeolian archipelago, namely: Lipari, Vulcano, Stromboli and Panarea. Samples were analyzed by H NMR profiling, and data were treated by PCA. Samples collected on Stromboli were very different from each other and from the samples collected in the other islands. Through an Orthogonal Partial Least Squares (OPLS) model, using altitude as the variable, it emerged that the concentration of proline, glycine betaine, sucrose, glucose and chlorogenic acid of growing on Stromboli decreased at increasing altitude. Conversely, increasing altitude was associated with an increment in valine, asparagine, fumaric acid and phenylalanine.
伊奥利亚群岛（意大利）是第勒尼安海中的一个火山群岛，由七个主要岛屿组成，其中有两座活火山。其独特的地质特征以及多样的环境和土壤对本地植物有重要影响，特别是伊奥利亚群岛上的（一种多年生冷季丛生禾草）种群表现出显著的表型变异性。鉴于环境驱动因素也强烈影响植物代谢物的产生，这项工作旨在比较在伊奥利亚群岛四个岛屿（即利帕里岛、武尔卡诺岛、斯特龙博利岛和帕纳雷阿岛）不同海拔采集的（叶片）代谢组学图谱。通过核磁共振氢谱分析对样本进行分析，并采用主成分分析（PCA）处理数据。在斯特龙博利岛采集的样本彼此之间以及与在其他岛屿采集的样本差异很大。通过使用海拔作为变量的正交偏最小二乘法（OPLS）模型发现，生长在斯特龙博利岛上的中脯氨酸、甘氨酸甜菜碱、蔗糖、葡萄糖和绿原酸的浓度随海拔升高而降低。相反，海拔升高与缬氨酸、天冬酰胺、富马酸和苯丙氨酸的增加有关。
