Lin Haiyan, Sun Jiayi, Hu Zhiwei, Cheng Chenxi, Lin Sue, Zou Huixi, Yan Xiufeng
National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Zhong-Xin Street, Wenzhou 325035, China.
Plants (Basel). 2022 Jun 14;11(12):1563. doi: 10.3390/plants11121563.
Glucosinolates (GLs) are plant secondary metabolites that may act against different types of cancers. Broccoli ( var. ) is rich in GLs which makes it an excellent source of these nutraceuticals. The composition and concentration of GLs vary among broccoli cultivars and throughout the developmental stages of the plant. To obtain the GL profiles of broccoli, GL compositions and contents in four early developmental stages (seeds, 3-day sprouts, 11-day and 17-day seedlings) were determined for nine cultivars of broccoli in this study. A total of 12 GLs including 9 aliphatic GLs and 3 indole GLs were identified from the nine broccoli cultivars using LC-QTOF-MS. UPLC results showed that aliphatic GLs concentrations decreased with broccoli sprouts and seedling growth for most cultivars. Interestingly, indole GLs amounts increased after germination and reached the highest level in 3-day sprouts or 11-day seedlings, and they fell back to a low level in 17-day seedlings. The GL profiles of nine cultivars documented in this study will provide useful information for high quality germplasm selection for cultivation or genetic engineering, and further understanding of the GL metabolic pathways.
硫代葡萄糖苷（GLs）是植物次生代谢产物，可能对不同类型的癌症起作用。西兰花（品种 ）富含硫代葡萄糖苷，这使其成为这些营养保健品的优质来源。硫代葡萄糖苷的组成和浓度在西兰花品种之间以及植物的整个发育阶段都有所不同。为了获得西兰花的硫代葡萄糖苷谱，本研究测定了9个西兰花品种在四个早期发育阶段（种子、3天龄芽苗、11天龄和17天龄幼苗）中的硫代葡萄糖苷组成和含量。使用液相色谱-四极杆飞行时间质谱（LC-QTOF-MS）从9个西兰花品种中总共鉴定出12种硫代葡萄糖苷，包括9种脂肪族硫代葡萄糖苷和3种吲哚族硫代葡萄糖苷。超高效液相色谱（UPLC）结果表明，对于大多数品种而言，随着西兰花芽苗和幼苗的生长，脂肪族硫代葡萄糖苷的浓度降低。有趣的是，吲哚族硫代葡萄糖苷的含量在发芽后增加，并在3天龄芽苗或11天龄幼苗中达到最高水平，而在17天龄幼苗中又降至低水平。本研究记录的9个品种的硫代葡萄糖苷谱将为栽培或基因工程的优质种质选择以及对硫代葡萄糖苷代谢途径的进一步了解提供有用信息。
