Wu Qiuyun, Mao Shuxiang, Huang Huiping, Liu Juan, Chen Xuan, Hou Linghui, Tian Yuxiao, Zhang Jiahui, Wang Junwei, Wang Yunsheng, Huang Ke
College of Horticulture, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan, 410128, China.
Hortic Res. 2024 Feb 28;11(5):uhae063. doi: 10.1093/hr/uhae063. eCollection 2024 May.
Broccoli ( var. Plenck) is an important vegetable crop, as it is rich in health-beneficial glucosinolates (GSLs). However, the genetic basis of the GSL diversity in Brassicaceae remains unclear. Here we report a chromosome-level genome assembly of broccoli generated using PacBio HiFi reads and Hi-C technology. The final genome assembly is 613.79 Mb in size, with a contig N50 of 14.70 Mb. The GSL profile and content analysis of different varieties, combined with a phylogenetic tree analysis, sequence alignment, and the construction of a 3D model of the methylthioalkylmalate synthase 1 (MAM1) protein, revealed that the gene copy number and amino acid sequence variation both contributed to the diversity of GSL biosynthesis in . The overexpression of (BolI0108790) in broccoli resulted in high accumulation and a high ratio of C4-GSLs, demonstrating that BoMAM1 is the key enzyme in C4-GSL biosynthesis. These results provide valuable insights for future genetic studies and nutritive component applications of crops.
西兰花（变种普伦克）是一种重要的蔬菜作物，因为它富含对健康有益的硫代葡萄糖苷（GSLs）。然而，十字花科中GSL多样性的遗传基础仍不清楚。在此，我们报告了利用PacBio HiFi reads和Hi-C技术生成的西兰花染色体水平的基因组组装。最终的基因组组装大小为613.79 Mb，重叠群N50为14.70 Mb。对不同品种的GSL谱和含量分析，结合系统发育树分析、序列比对以及甲硫基烷基苹果酸合酶1（MAM1）蛋白三维模型的构建，揭示了基因拷贝数和氨基酸序列变异均导致了西兰花中GSL生物合成的多样性。西兰花中BoMAM1（BolI0108790）的过表达导致C4-GSLs的高积累和高比例，表明BoMAM1是C4-GSL生物合成中的关键酶。这些结果为未来十字花科作物的遗传研究和营养成分应用提供了有价值的见解。
