College of Life Sciences, China Jiliang University, Hangzhou, 310018, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
J Insect Sci. 2022 May 1;22(3). doi: 10.1093/jisesa/ieac041.
Drosophila E74 is an early gene located in the polytene chromosome 74EF puff position. E74 controls the production of late genes, indicating that it plays a crucial role in this cascade model. Nilaparvata lugens E74 is closely related to Diaphorina citri, Bemisia tabaci, and Laodelphax striatellus. After downregulating E74, molting, and nymphal mortality were increased, and ovarian development was delayed. Moreover, the expression of Vg was reduced at the transcriptional level, as measured by qRT-PCR, and the content of Vg protein was reduced, as detected by Western blotting. After downregulating E74, the expression of hormone-related genes, including Tai, βFtz-F1, Met, Kr-h1, UspA, UspB, E93, and Br, was changed. The expression of E74 was significantly decreased after downregulating hormone-related genes. When the expression of E74 and βFtz-F1 was downregulated together, nymph mortality and molting mortality were higher than those when E74 or βFtz-F1 was downregulated alone. Thus, E74 probably interacts with βFtz-F1 at the genetic level. In summary, this study showed that E74 plays a crucial role in the development, metamorphosis and reproduction of N. lugens, possibly via the interaction with βFtz-F1 at the genetic level. This study provides a basis for the development of new target-based pesticides and new methods for the effective control of N. lugens.
果蝇 E74 是一个早期基因，位于多线染色体 74EF 膨大区位置。E74 控制晚期基因的产生，表明它在这个级联模型中起着至关重要的作用。褐飞虱 E74 与烟粉虱、棉蚜和灰飞虱密切相关。下调 E74 后，蜕皮和若虫死亡率增加，卵巢发育延迟。此外，通过 qRT-PCR 测量，Vg 的转录水平降低，Western blot 检测到 Vg 蛋白含量减少。下调 E74 后，包括 Tai、βFtz-F1、Met、Kr-h1、UspA、UspB、E93 和 Br 在内的激素相关基因的表达发生变化。下调激素相关基因后，E74 的表达显著降低。当同时下调 E74 和βFtz-F1 的表达时，若虫死亡率和蜕皮死亡率高于单独下调 E74 或βFtz-F1 时的死亡率。因此，E74 可能在遗传水平上与βFtz-F1 相互作用。总之，本研究表明 E74 在褐飞虱的发育、变态和生殖中起着至关重要的作用，可能通过与βFtz-F1 在遗传水平上的相互作用来实现。本研究为开发新的基于靶标的杀虫剂和有效控制褐飞虱的新方法提供了依据。
