State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China.
J Adv Res. 2022 Dec;42:55-67. doi: 10.1016/j.jare.2022.06.007. Epub 2022 Jun 20.
Cotton is a vital industrial crop that is gradually shifting to planting in arid areas. However, tubby-like proteins (TULPs) involved in plant response to various stresses are rarely reported in cotton. The present study exhibited that GhTULP30 transcription in cotton was induced by drought stress.
The present study demonstrated the improvement of plant tolerance to drought stress by GhTULP30 through regulation of stomatal movement.
GhTULP30 response to drought and salt stress was preliminarily confirmed by qRT-PCR and yeast stress experiments. Ectopic expression in Arabidopsis and endogenous gene silencing in cotton were used to determine stomatal movement. Yeast two-hybrid and spilt-luciferase were used to screen the interacting proteins.
Ectopic expression of GhTULP30 in yeast markedly improved yeast cell tolerance to salt and drought. Overexpression of GhTULP30 made Arabidopsis seeds more resistant to drought and salt stress during seed germination and increased the stomata closing speed of the plant under drought stress conditions. Silencing of GhTULP30 in cotton by virus-induced gene silencing (VIGS) technology slowed down the closure speed of stomata under drought stress and decreased the length and width of the stomata. The trypan blue and diaminobenzidine staining exhibited the severity of leaf cell necrosis of GhTULP30-silenced plants. Additionally, the contents of proline, malondialdehyde, and catalase of GhTULP30-silenced plants exhibited significant variations, with obvious leaf wilting. Protein interaction experiments exhibited the interaction of GhTULP30 with GhSKP1B and GhXERICO.
GhTULP30 participates in plant response to drought stress. The present study provides a reference and direction for further exploration of TULP functions in cotton plants.
棉花是一种重要的工业作物，正逐渐向干旱地区种植转移。然而，参与植物对各种胁迫反应的管状蛋白（TULP）在棉花中很少有报道。本研究表明，棉花中 GhTULP30 的转录受到干旱胁迫的诱导。
本研究通过调节气孔运动，证明 GhTULP30 可提高植物对干旱胁迫的耐受性。
通过 qRT-PCR 和酵母应激实验初步证实 GhTULP30 对干旱和盐胁迫的反应。在拟南芥中异位表达和棉花中内源基因沉默用于确定气孔运动。酵母双杂交和分裂荧光素酶用于筛选互作蛋白。
酵母中 GhTULP30 的异位表达显著提高了酵母细胞对盐和干旱的耐受性。GhTULP30 的过表达使拟南芥种子在种子萌发过程中对干旱和盐胁迫更具抗性，并增加了植物在干旱胁迫条件下气孔关闭的速度。通过病毒诱导基因沉默（VIGS）技术沉默棉花中的 GhTULP30 会减缓气孔在干旱胁迫下的关闭速度，并降低气孔的长度和宽度。台盼蓝和二氨基联苯的染色显示了 GhTULP30 沉默植物叶片细胞坏死的严重程度。此外，GhTULP30 沉默植物的脯氨酸、丙二醛和过氧化氢酶含量也发生了显著变化，叶片明显萎蔫。蛋白相互作用实验表明 GhTULP30 与 GhSKP1B 和 GhXERICO 相互作用。
GhTULP30 参与植物对干旱胁迫的反应。本研究为进一步探索 TULP 在棉花中的功能提供了参考和方向。
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