Wang Qing, Guo Tongtong, Yi Yuxiao, Yin Zelin, Xu Lili, Yang Fengtang, Xu Jianing
School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, China.
BMC Plant Biol. 2025 May 10;25(1):618. doi: 10.1186/s12870-025-06643-2.
Drought and salt stresses as common abiotic stresses have a significant impact on the yield and quality of Malus domestica (apple). The tubby-like proteins (TLPs), as a family of transcription factors, play an important role in plant response to abiotic stresses, but the regulatory mechanisms involved remain unclear.
Here, we investigated how apple TLP7 (MdTLP7) and its interacting genes regulate apple response to drought and salt stresses. The results shows that overexpression of MdTLP7 significantly increased the drought and salt tolerance of apple through regulating gene transcription. Yeast two-hybrid screening, together with DNA affinity purification sequencing (DAP-seq), revealed that MdTLP7 interacted with MdNAC72L to regulate the expression of MdHsp70-8 and MdAGP, and that the overexpression of these two genes significantly enhanced the drought and salt stresses tolerance of apple.
The apple protein MdTLP7 could interact with MdNAC72L and promoted the expression of MdHsp70-8 and MdAGP, thus improved drought and salt tolerance of apple calli. These results provide insights into the molecular mechanism of MdTLP7 increasing the drought and salt stresses tolerance of apple as well as a theoretical basis for the expansion of apple cultivation and related research.
干旱和盐胁迫作为常见的非生物胁迫，对苹果的产量和品质有重大影响。类筒状蛋白（TLP）作为一类转录因子，在植物对非生物胁迫的响应中发挥重要作用，但其涉及的调控机制尚不清楚。
在此，我们研究了苹果TLP7（MdTLP7）及其相互作用基因如何调控苹果对干旱和盐胁迫的响应。结果表明，MdTLP7的过表达通过调控基因转录显著提高了苹果的耐旱性和耐盐性。酵母双杂交筛选以及DNA亲和纯化测序（DAP-seq）表明，MdTLP7与MdNAC72L相互作用以调控MdHsp70-8和MdAGP的表达，并且这两个基因的过表达显著增强了苹果的耐旱性和耐盐性。
苹果蛋白MdTLP7可与MdNAC72L相互作用并促进MdHsp70-8和MdAGP的表达，从而提高苹果愈伤组织的耐旱性和耐盐性。这些结果为MdTLP7提高苹果耐旱性和耐盐性的分子机制提供了见解，并为苹果栽培扩展及相关研究提供了理论依据。
