Key Laboratory of Ecological Adaptation and Evolution of Extreme Environment in Xinjiang, College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, China.
College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China.
Genes (Basel). 2024 Aug 12;15(8):1063. doi: 10.3390/genes15081063.
The SEVEN IN ABSENTIA (SINA) E3 ubiquitin ligase is widely involved in drought and salt stress in plants. However, the biological function of the SINA proteins in cotton is still unknown. This study aimed to reveal the function of through biochemical, genetic and molecular approaches. is expressed in several tissues of cotton plants, including roots, stems, leaves and cotyledons, and its expression levels are significantly affected by polyethylene glycol, abscisic acid and sodium chloride. When was silenced in cotton plants, drought and salinity stress occurred, and the length, area and volume of the roots significantly decreased. Under drought stress, the levels of proline, superoxide dismutase, peroxidase and catalase in the -silenced cotton plants were significantly lower than those in the non-silenced control plants, whereas the levels of hydrogen peroxide and malondialdehyde were greater. Moreover, the expression of stress-related genes in silenced plants under drought stress suggested that may play a positive role in the plant response to drought and salt stress by regulating these stress response-related genes. These findings not only deepen our understanding of the mechanisms of drought resistance in cotton but also provide potential targets for future improvements in crop stress resistance through genetic engineering.
缺失的 SEVEN IN ABSENTIA (SINA) E3 泛素连接酶广泛参与植物的干旱和盐胁迫。然而，SINA 蛋白在棉花中的生物学功能尚不清楚。本研究旨在通过生化、遗传和分子方法揭示 的功能。 在棉花植物的几种组织中表达，包括根、茎、叶和子叶，其表达水平受聚乙二醇、脱落酸和氯化钠的显著影响。当 在棉花植株中沉默时，干旱和盐胁迫发生，根的长度、面积和体积显著减小。在干旱胁迫下，沉默棉花植株中的脯氨酸、超氧化物歧化酶、过氧化物酶和过氧化氢酶的水平明显低于非沉默对照植株，而过氧化氢和丙二醛的水平则更高。此外，干旱胁迫下沉默植株中与应激相关的基因的表达表明， 通过调节这些应激反应相关基因，可能在植物对干旱和盐胁迫的反应中发挥积极作用。这些发现不仅加深了我们对棉花抗旱机制的理解，还为通过遗传工程提高作物抗胁迫能力提供了潜在的目标。
