Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Pfizer Vaccine Research and Development, Pearl River, New York, NY, USA.
Nat Commun. 2022 Jun 23;13(1):3602. doi: 10.1038/s41467-022-30681-1.
The newly emerged Omicron SARS-CoV-2 has several distinct sublineages including BA.1, BA.2, and BA.3. BA.1 accounts for the initial surge and is being replaced by BA.2, whereas BA.3 is at a low prevalence at this time. Here we report the neutralization of BNT162b2-vaccinated sera (collected 1 month after dose 3) against the three Omicron sublineages. To facilitate the neutralization testing, we have engineered the complete BA.1, BA.2, or BA.3 spike into an mNeonGreen USA-WA1/2020 SRAS-CoV-2. All BNT162b2-vaccinated sera neutralize USA-WA1/2020, BA.1-, BA.2-, and BA.3-spike SARS-CoV-2s with titers of >20; the neutralization geometric mean titers (GMTs) against the four viruses are 1211, 336, 300, and 190, respectively. Thus, the BA.1-, BA.2-, and BA.3-spike SARS-CoV-2s are 3.6-, 4.0-, and 6.4-fold less efficiently neutralized than the USA-WA1/2020, respectively. Our data have implications in vaccine strategy and understanding the biology of Omicron sublineages.
新出现的奥密克戎 SARS-CoV-2 有几个不同的亚谱系，包括 BA.1、BA.2 和 BA.3。BA.1 导致了最初的激增，正在被 BA.2 取代，而 BA.3 此时的流行率较低。在这里，我们报告了 BNT162b2 疫苗接种血清（在第 3 剂后 1 个月采集）对三种奥密克戎亚谱系的中和作用。为了便于中和试验，我们将完整的 BA.1、BA.2 或 BA.3 刺突蛋白工程化为 mNeonGreen USA-WA1/2020 SRAS-CoV-2。所有 BNT162b2 疫苗接种血清均能中和 USA-WA1/2020、BA.1-、BA.2-和 BA.3-刺突 SARS-CoV-2，滴度>20；针对这四种病毒的中和几何平均滴度（GMT）分别为 1211、336、300 和 190。因此，BA.1-、BA.2-和 BA.3-刺突 SARS-CoV-2 的中和效率分别比 USA-WA1/2020 低 3.6 倍、4.0 倍和 6.4 倍。我们的数据对疫苗策略和理解奥密克戎亚谱系的生物学具有重要意义。
