Bekiari Chryssa, Tekos Fotios, Skaperda Zoi, Argyropoulou Aikaterini, Skaltsounis Alexios-Leandros, Kouretas Demetrios, Tsingotjidou Anastasia
Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Laboratory of Animal Physiology, Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece.
Antioxidants (Basel). 2022 May 27;11(6):1062. doi: 10.3390/antiox11061062.
Oxaliplatin is a widely used chemotherapeutic agent. Despite its many beneficial aspects in fighting many malignancies, it shares an aversive effect of neuropathy. Many substances have been used to limit this oxaliplatin-driven neuropathy in patients. This study evaluates the neuroprotective role of a grape pomace extract (GPE) into an oxaliplatin induced neuropathy in rats. For this reason, following the delivery of the substance into the animals prior to or simultaneously with oxaliplatin, their performance was evaluated by behavioral tests. Blood tests were also performed for the antioxidant activity of the extract, along with a histological and pathological evaluation of dorsal root ganglion (DRG) cells as the major components of the neuropathy. All behavioral tests were corrected following the use of the grape pomace. Oxidative stressors were also limited with the use of the extract. Additionally, the morphometrical analysis of the DRG cells and their immunohistochemical phenotype revealed the fidelity of the animal model and the changes into the parvalbumin and GFAP concentration indicative of the neuroprotective role of the pomace. In conclusion, the grape pomace extract with its antioxidant properties alleviates the harmful effects of the oxaliplatin induced chronic neuropathy in rats.
奥沙利铂是一种广泛使用的化疗药物。尽管它在对抗多种恶性肿瘤方面有诸多益处，但它也会产生令人厌恶的神经病变副作用。许多物质已被用于减轻患者因奥沙利铂引起的神经病变。本研究评估了葡萄皮渣提取物（GPE）对大鼠奥沙利铂诱导的神经病变的神经保护作用。因此，在给动物注射奥沙利铂之前或同时给予该物质后，通过行为测试评估它们的表现。还对提取物的抗氧化活性进行了血液检测，并对作为神经病变主要组成部分的背根神经节（DRG）细胞进行了组织学和病理学评估。使用葡萄皮渣后，所有行为测试结果均得到改善。提取物的使用还限制了氧化应激。此外，DRG细胞的形态计量分析及其免疫组化表型揭示了动物模型的可靠性，以及小白蛋白和胶质纤维酸性蛋白浓度的变化，表明了皮渣的神经保护作用。总之，具有抗氧化特性的葡萄皮渣提取物减轻了奥沙利铂诱导的大鼠慢性神经病变的有害影响。
