Abderrahim Amel, Djahmi Nassima, Loucif Lotfi, Nedjai Sabrina, Chelaghma Widad, Gameci-Kirane Djamila, Dekhil Mazouz, Lavigne Jean-Philippe, Pantel Alix
Département de Biochimie, Faculté des Sciences, Université Badji Mokhtar Annaba, Annaba 23000, Algeria.
Laboratoire de Microbiologie, CHU Ibn Rochd, Annaba 23000, Algeria.
Antibiotics (Basel). 2022 May 31;11(6):750. doi: 10.3390/antibiotics11060750.
Multidrug-resistant (MDR) Enterobacterales remain an increasing problem in Algeria, notably due to the emergence of carbapenemase producers. We investigated the molecular characteristics of carbapenem-resistant Enterobacterales isolates recovered from outpatients and inpatients in Eastern Algeria. Non-repetitive Enterobacterales with reduced susceptibility to carbapenems were consecutively collected from clinical specimens in Annaba University Hospital (Algeria) between April 2016 and December 2018. Isolates were characterized with regard to antibiotic resistance, resistome and virulome content, clonality, and plasmid support. Of the 168 isolates analyzed, 29 (17.3%) were carbapenemase producers and identified as ( = 23), ( = 5), and ( = 1). was the most prevalent carbapenemase-encoding gene ( = 26/29), followed by gene ( = 3/29). isolates harbored some virulence traits (, , , , ), whereas had a commensal origin (E, A, and B1). Clonality analysis revealed clonal expansions of ST101 and ST758 . Plasmid analysis showed a large diversity of incompatibility groups, with a predominance of IncM ( = 26, 89.7%). A global dissemination of OXA-48-producing Enterobacterales in the Algerian hospital but also the detection of NDM-1-producing in community settings were observed. The importance of this diffusion must be absolutely investigated and controlled.
多重耐药（MDR）肠杆菌科细菌在阿尔及利亚仍然是一个日益严重的问题，尤其是由于碳青霉烯酶产生菌的出现。我们调查了从阿尔及利亚东部门诊和住院患者中分离出的耐碳青霉烯肠杆菌科细菌的分子特征。2016年4月至2018年12月期间，在安纳巴大学医院（阿尔及利亚）从临床标本中连续收集对碳青霉烯类药物敏感性降低的非重复性肠杆菌科细菌。对分离株进行了抗生素耐药性、耐药基因组和毒力基因组含量、克隆性及质粒支持方面的特征分析。在分析的168株分离株中，29株（17.3%）为碳青霉烯酶产生菌，鉴定为 blaOXA-48（n = 23）、blaNDM-1（n = 5）和 blaVIM（n = 1）。blaOXA-48是最常见的碳青霉烯酶编码基因（26/29），其次是 blaNDM-1基因（3/29）。blaOXA-48分离株具有一些毒力特征（fimH、papC、sfa/foc、kpsMT II和ibeA），而blaNDM-1具有共生起源（E、A和B1）。克隆性分析显示ST101 blaOXA-48和ST758 blaNDM-1存在克隆扩增。质粒分析显示不相容群具有很大的多样性，以IncM为主（n = 26，89.7%）。在阿尔及利亚医院观察到产OXA-48肠杆菌科细菌的全球传播，同时在社区环境中也检测到产NDM-1肺炎克雷伯菌。必须对这种传播的重要性进行彻底调查和控制。
