Chang Jung-Wei, Chen Hsin-Chang, Hu Heng-Zhao, Chang Wan-Ting, Huang Po-Chin, Wang I-Jen
Institute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan.
Department of Chemistry, Tunghai University, Taichung 407224, Taiwan.
Biomedicines. 2022 Jun 17;10(6):1438. doi: 10.3390/biomedicines10061438.
Whether low-dose phthalate exposure triggers asthma among children, and its underlying mechanisms, remain debatable. Here, we evaluated the individual and mixed effects of low-dose phthalate exposure on children with asthma and five (oxidative/nitrosative stress/lipid peroxidation) mechanistic biomarkers-8-hydroxy-2'-deoxyguanosine (8-OHdG), 8-nitroguanine (8-NOGua), 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA), 8-isoprostaglandin F2α (8-isoPF2α), and malondialdehyde (MDA)-using a propensity score-matched case-control study (case vs. control = 41 vs. 111). The median monobenzyl phthalate (MBzP) concentrations in the case group were significantly higher than those in the control group (3.94 vs. 2.52 ng/mL, = 0.02), indicating that dust could be an important source. After adjustment for confounders, the associations of high monomethyl phthalate (MMP) (75th percentile) with 8-NOGua (adjusted odds ratio (aOR): 2.66, 95% confidence interval (CI): 1.03-6.92) and 8-isoPF2α (aOR: 4.04, 95% CI: 1.51-10.8) and the associations of mono-iso-butyl phthalate (MiBP) with 8-isoPF2α (aOR: 2.96, 95% CI: 1.13-7.79) were observed. Weighted quantile sum regression revealed that MBzP contributed more than half of the association (56.8%), followed by MiBP (26.6%) and mono-iso-nonyl phthalate (MiNP) (8.77%). Our findings supported the adjuvant effect of phthalates in enhancing the immune system response.
低剂量邻苯二甲酸盐暴露是否会引发儿童哮喘及其潜在机制仍存在争议。在此，我们采用倾向评分匹配的病例对照研究（病例组与对照组 = 41对111），评估了低剂量邻苯二甲酸盐暴露对哮喘儿童的个体和混合效应，以及五种（氧化/亚硝化应激/脂质过氧化）机制生物标志物——8-羟基-2'-脱氧鸟苷（8-OHdG）、8-硝基鸟嘌呤（8-NOGua）、4-羟基-2-壬烯醛-巯基尿酸（HNE-MA）、8-异前列腺素F2α（8-isoPF2α）和丙二醛（MDA）的影响。病例组中邻苯二甲酸单苄酯（MBzP）的中位数浓度显著高于对照组（3.94对2.52 ng/mL，P = 0.02），表明灰尘可能是一个重要来源。在对混杂因素进行调整后，观察到高邻苯二甲酸单甲酯（MMP）（第75百分位数）与8-NOGua（调整后的优势比（aOR）：2.66，95%置信区间（CI）：1.03 - 6.92）和8-isoPF2α（aOR：4.04，95% CI：1.51 - 10.8）之间的关联，以及邻苯二甲酸单异丁酯（MiBP）与8-isoPF2α（aOR：2.96，95% CI：1.13 - 7.79）之间存在关联。加权分位数和回归显示，MBzP对关联的贡献超过一半（56.8%），其次是MiBP（26.6%）和邻苯二甲酸单异壬酯（MiNP）（8.77%）。我们的研究结果支持了邻苯二甲酸盐在增强免疫系统反应方面的辅助作用。
