Bahri Racha, Boyer François, Halabi Mohamad Adnan, Chaunavel Alain, Feuillard Jean, Jaccard Arnaud, Ranger-Rogez Sylvie
Microbiology Department, Faculty of Pharmacy, 87025 Limoges, France.
Unité Mixte de Recherche CNRS (Centre National de la Recherche Scientifique) 7276 and INSERM (Institut National de la Santé et de la Recherche Médicale) U1262, 87025 Limoges, France.
Cancers (Basel). 2022 Jun 12;14(12):2899. doi: 10.3390/cancers14122899.
The Epstein-Barr virus (EBV) is associated with angioimmunoblastic T cell lymphoma (AITL), a peripheral T lymphoma of poor prognosis in at least 90% of cases. The role of EBV in this pathology is unknown. Using next-generation sequencing, we sequenced the entire EBV genome in biopsies from 18 patients with AITL, 16 patients with another EBV-associated lymphoma, and 2 controls. We chose an EBV target capture method, given the high specificity of this technique, followed by a second capture to increase sensitivity. We identified two main viral strains in AITL, one of them associated with the mutations S542N and A206S and with mutations in the and genes. This strain was characterized in patients with short post-diagnosis survival. The main mutations found during AITL on the most mutated latency or tegument genes were identified and discussed. We showed that the virus was clonal in all the AITL samples, suggesting that it may be involved in this pathology. Additionally, EBV was latent in all the AITL samples; for one sample only, the virus was found to be latent and probably replicative, depending on the cells. These various elements support the role of EBV in AITL.
爱泼斯坦-巴尔病毒（EBV）与血管免疫母细胞性T细胞淋巴瘤（AITL）相关，AITL是一种外周T细胞淋巴瘤，至少90%的病例预后较差。EBV在这种病理状态中的作用尚不清楚。我们使用下一代测序技术，对18例AITL患者、16例其他EBV相关淋巴瘤患者的活检样本以及2例对照的整个EBV基因组进行了测序。鉴于该技术的高特异性，我们选择了EBV靶向捕获方法，随后进行第二次捕获以提高灵敏度。我们在AITL中鉴定出两种主要病毒株，其中一种与S542N和A206S突变以及 和 基因的突变相关。该毒株在诊断后生存期短的患者中具有特征性。我们对AITL中在最易发生突变的潜伏或被膜基因上发现的主要突变进行了鉴定和讨论。我们发现，在所有AITL样本中病毒都是克隆性的，这表明它可能与这种病理状态有关。此外，在所有AITL样本中EBV都处于潜伏状态；仅在一个样本中，根据细胞情况发现病毒处于潜伏状态且可能具有复制性。这些不同的因素支持了EBV在AITL中的作用。
