Vargas-Alarcón Gilberto, Ramírez-Bello Julian, Peña-Duque Marco Antonio, Martínez-Ríos Marco Antonio, Delgadillo-Rodríguez Hilda, Fragoso José Manuel
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City 14080, Mexico.
Department of Endocrinology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City 14080, Mexico.
Biomolecules. 2022 May 31;12(6):765. doi: 10.3390/biom12060765.
In the present study, we evaluated the association of the BAT1, NFKBIL, LTA, and CASP1 single nucleotide polymorphisms and the gene−gene interactions with risk of developing restenosis after coronary stenting. The allele and genotype determination of the polymorphisms (BAT1 rs2239527 C/G, NFKBIL1 rs2071592 T/A, LTA rs1800683 G/A, CASP1 rs501192 A/G, and CASP1 rs580253 A/G) were performed by 5’exonuclease TaqMan assays in 219 patients: 66 patients with restenosis and 153 without restenosis. The distribution of rs2239527 C/G, rs2071592 T/A, and rs1800683 G/A polymorphisms was similar in patients with and without restenosis. Nonetheless, under recessive (OR = 2.73, pCRes = 0.031) and additive models (OR = 1.65, pCAdd = 0.039), the AA genotype of the rs501192 A/G polymorphism increased the restenosis risk. Under co-dominant, dominant, recessive, and additive models, the AA genotype of the rs580253 A/G was associated with a high restenosis risk (OR = 5.38, pCCo-Dom = 0.003; OR = 2.12, pCDom = 0.031; OR = 4.32, pCRes = 0.001; and OR = 2.16, 95%CI: 1.33−3.52, pCAdd = 0.001, respectively). In addition, we identified an interaction associated with restenosis susceptibility: BAT1-NFKBIL1-LTA-CASP1 (OR = 9.92, p < 0.001). In summary, our findings demonstrate that the rs501192 A/G and rs580253 A/G polymorphisms, as well as the gene−gene interactions between BAT1-NFKBIL1-LTA-CASP1, are associated with an increased restenosis risk after coronary stenting.
在本研究中，我们评估了BAT1、NFKBIL、LTA和CASP1单核苷酸多态性以及基因-基因相互作用与冠状动脉支架置入术后再狭窄风险的关联。通过5'外切核酸酶TaqMan分析对219例患者（66例再狭窄患者和153例无再狭窄患者）进行多态性（BAT1 rs2239527 C/G、NFKBIL1 rs2071592 T/A、LTA rs1800683 G/A、CASP1 rs501192 A/G和CASP1 rs580253 A/G）的等位基因和基因型测定。rs2239527 C/G、rs2071592 T/A和rs1800683 G/A多态性在有再狭窄和无再狭窄患者中的分布相似。然而，在隐性模型（OR = 2.73，pCRes = 0.031）和加性模型（OR = 1.65，pCAdd = 0.039）下，rs501192 A/G多态性的AA基因型增加了再狭窄风险。在共显性、显性、隐性和加性模型下，rs580253 A/G的AA基因型与高再狭窄风险相关（分别为OR = 5.38，pCCo-Dom = 0.003；OR = 2.12，pCDom = 0.031；OR = 4.32，pCRes = 0.001；OR = 2.
