Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
Biomolecules. 2022 Jun 1;12(6):771. doi: 10.3390/biom12060771.
Electric-toothbrush vibrations, which remove plaque, are transmitted to the gingival connective tissue via epithelial cells. Physical energy affects cell function; however, the effects of electric-toothbrush vibrations on gingival extracellular matrix (ECM) protein expression remain unknown. We aimed to examine the effects of these vibrations on the expression of ECM proteins-type I collagen (col I), type III collagen (col III), elastin, and fibronectin (FN)-using human gingival fibroblasts (HGnFs). HGnFs were seeded for 5 days in a six-well plate with a hydrophilic surface, exposed to electric-toothbrush vibrations, and cultured for 7 days. Subsequently, the mRNA and protein levels of col I, col III, elastin, and FN were examined. To investigate the role of focal adhesion kinase (FAK) signaling on ECM protein expression in vibration-stimulated cells, the cells were treated with siRNA against protein tyrosine kinase (PTK). Electric-toothbrush vibrations increased col I, col III, elastin, and FN expression; promoted collagen and non-collagen protein production; and enhanced FAK phosphorylation in HGnFs. Moreover, PTK2 siRNA completely blocked the effects of these vibrations on the expression of col I, col III and elastin mRNA. The results suggest that electric-toothbrush vibrations increase collagen, elastin, and FN production through the FAK-signaling pathway in fibroblasts.
电动牙刷的振动通过上皮细胞传递到牙龈结缔组织，从而去除牙菌斑。物理能量会影响细胞功能；然而，电动牙刷振动对牙龈细胞外基质 (ECM) 蛋白表达的影响尚不清楚。我们旨在使用人牙龈成纤维细胞 (HGnFs) 研究这些振动对 ECM 蛋白表达的影响，这些蛋白包括 I 型胶原 (col I)、III 型胶原 (col III)、弹性蛋白和纤维连接蛋白 (FN)。将 HGnFs 接种到具有亲水表面的六孔板中培养 5 天，然后暴露于电动牙刷振动中培养 7 天。随后，检测 col I、col III、弹性蛋白和 FN 的 mRNA 和蛋白水平。为了研究粘着斑激酶 (FAK) 信号通路在振动刺激细胞中对 ECM 蛋白表达的作用，用针对蛋白酪氨酸激酶 (PTK) 的 siRNA 处理细胞。电动牙刷振动增加了 col I、col III、弹性蛋白和 FN 的表达；促进了胶原蛋白和非胶原蛋白的产生；并增强了 HGnFs 中的 FAK 磷酸化。此外，PTK2 siRNA 完全阻断了这些振动对 col I、col III 和弹性蛋白 mRNA 表达的影响。结果表明，电动牙刷振动通过成纤维细胞中的 FAK 信号通路增加了胶原蛋白、弹性蛋白和 FN 的产生。
