Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 101-0061, Japan.
Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo 101-0061, Japan.
Biomolecules. 2022 Jun 20;12(6):857. doi: 10.3390/biom12060857.
Each growth factor (GF) has different effects and targets, and plays a critical role in periodontal healing. Dehydrated human amnion-chorion membrane (dHACM) contains various GFs and has been used to enhance wound healing. The purpose of this study was to evaluate the effects of dHACM on periodontal healing, using in vitro and in vivo experimental approaches. Standardized periodontal defects were created in rats. The defects were randomly divided into three groups: Unfilled, filled with hydroxypropyl cellulose (HPC), and dHACM+HPC. At 2 and 4 weeks postoperatively, periodontal healing was analyzed by microcomputed tomography (micro-CT), and histological and immunohistochemical analyses. In vitro, periodontal ligament-derived cells (PDLCs) isolated from rat incisors were incubated with dHACM extract. Cell proliferation and migration were evaluated by WST-1 and wound healing assay. In vivo, micro-CT examination at 2 weeks revealed enhanced formation of new bone in the dHACM+HPC group. At 4 weeks, the proportions of vascular endothelial growth factor (VEGF)-positive cells and α-smooth muscle actin (α-SMA)-positive blood vessels in the dHACM+HPC group were significantly greater than those in the Unfilled group. In vitro, dHACM extracts at 100 µg/mL significantly increased cell proliferation and migration compared with control. These findings suggest that GFs contained in dHACM promote proliferation and migration of PDLCs and angiogenesis, which lead to enhanced periodontal healing.
每种生长因子（GF）都有不同的作用和靶点，在牙周愈合中起着关键作用。脱水人羊膜 - 绒毛膜膜（dHACM）含有各种 GF，并已被用于增强伤口愈合。本研究的目的是通过体外和体内实验方法评估 dHACM 对牙周愈合的影响。在大鼠中创建标准化的牙周缺损。将缺陷随机分为三组：未填充，填充羟丙基纤维素（HPC）和 dHACM+HPC。术后 2 和 4 周，通过 microCT（micro-CT）和组织学及免疫组织化学分析分析牙周愈合情况。在体外，从大鼠切牙分离的牙周韧带衍生细胞（PDLCs）与 dHACM 提取物孵育。通过 WST-1 和划痕愈合测定评估细胞增殖和迁移。在体内，2 周时的 micro-CT 检查显示 dHACM+HPC 组中形成了更多的新骨。4 周时，dHACM+HPC 组中血管内皮生长因子（VEGF）阳性细胞和α-平滑肌肌动蛋白（α-SMA）阳性血管的比例明显高于未填充组。在体外，与对照组相比，100μg/mL 的 dHACM 提取物显著增加了细胞增殖和迁移。这些发现表明，dHACM 中包含的 GF 促进了 PDLC 的增殖和迁移以及血管生成，从而增强了牙周愈合。
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