Haller Nils, Tomaskovic Aleksandar, Stöggl Thomas, Simon Perikles, Neuberger Elmo
Department of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University of Mainz, 55099 Mainz, Germany.
Department of Sport and Exercise Science, University of Salzburg, 5400 Rif, Austria.
Diagnostics (Basel). 2022 Jun 2;12(6):1379. doi: 10.3390/diagnostics12061379.
Circulating, cell-free DNA (cfDNA) has been discussed as an upcoming blood-based biomarker in exercise physiology, reflecting important aspects of exercise load. cfDNA blood sampling has evolved from elaborate venous to efficient capillary sampling from the fingertips. In this study, we aimed to evaluate the principal feasibility of cfDNA blood sampling from the earlobe. Therefore, we obtained cfDNA concentrations from the fingertips, earlobe, and the antecubital vein during physiological exercise testing. Significantly higher concentrations were obtained from the earlobe compared to fingertip samples. All of the measurement methods showed good to excellent repeatability (ICCs of 0.85 to 0.93). In addition, the control experiments revealed that repeated sampling from the earlobe but not from the fingertips increased cfDNA at rest. In summary, cfDNA sampling is feasible for all sampling sources. However, at rest, cfDNA collected from the earlobe tend to increase over time in the absence of physical load, potentially limiting this sampling method.
循环游离DNA（cfDNA）作为运动生理学中一种新兴的血液生物标志物，已被广泛讨论，它反映了运动负荷的重要方面。cfDNA血液采样已从复杂的静脉采样发展为从指尖进行高效的毛细血管采样。在本研究中，我们旨在评估从耳垂采集cfDNA血液样本的主要可行性。因此，我们在生理运动测试期间从指尖、耳垂和肘前静脉获取了cfDNA浓度。与指尖样本相比，从耳垂获得的浓度显著更高。所有测量方法均显示出良好到优异的重复性（组内相关系数为0.85至0.93）。此外，对照实验表明，在静息状态下，重复从耳垂而非指尖采样会增加cfDNA。总之，cfDNA采样对于所有采样来源都是可行的。然而，在静息状态下，在没有身体负荷的情况下，从耳垂采集的cfDNA可能会随着时间推移而增加，这可能会限制这种采样方法。
