盘基网柄菌变形虫与小鼠成纤维细胞之间通过聚乙二醇诱导形成的胞质杂种和异核体。
Cybrids and heterokaryons PEG-induced between D. discoideum amoebae and mouse fibroblasts.
作者信息
Fighetti M A, Rubino S, Arena N, Cappuccinelli P
出版信息
Microbiologica. 1987 Jan;10(1):9-18.
PMID:3574145
Abstract
Optimal conditions for fusion of Dictyostelium discoideum amoebae with mouse fibroblasts using polyethylene glycol (PEG) are described. Three different kinds of experiments were done to look for the ways to obtain new cell lines heterokaryons or cybrids. In all cases, the final products of the fusion were cells showing new features, still viable for at least one week. To detect the cell membrane fusion, samples were observed by scanning electron microscope (SEM).
摘要
描述了使用聚乙二醇（PEG）使盘基网柄菌变形虫与小鼠成纤维细胞融合的最佳条件。进行了三种不同类型的实验，以寻找获得新细胞系（异核体或胞质杂种）的方法。在所有情况下，融合的最终产物都是表现出新特征的细胞，并且至少存活了一周。为了检测细胞膜融合，通过扫描电子显微镜（SEM）观察样品。
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