Wan Meixia, Li Qing, Lei Qianya, Zhou Dan, Wang Shu
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Qibo College of Medicine, Longdong University, Qingyang 745000, China.
Foods. 2022 Jun 20;11(12):1818. doi: 10.3390/foods11121818.
L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS). However, both the protective effects and mechanisms of different fractions extracted from L. fruit against MetS remain unclear. Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS. This study aimed to investigate the potential role of polyphenols and polysaccharides derived from L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles. In the study, polyphenols and polysaccharides derived from L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress. In particular, the group treated with polyphenols and polysaccharides in combination showed better efficacy. The relative abundance of beneficial bacterial genera and were increased to various degrees, while opportunistic pathogens such as , , and were markedly decreased after treatments. Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from L. fruit, individually and in combination. Altogether, these results demonstrated that polyphenols and polysaccharides derived from L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
L.果是东亚一种药食两用的水果，对代谢综合征（MetS）显示出潜在的健康促进作用。然而，从L.果中提取的不同组分对MetS的保护作用及其机制仍不清楚。此外，肠道微生物群及其代谢产物被认为是MetS发生发展的关键因素。本研究旨在探讨L.果中多酚和多糖对高脂饮食（HFD）喂养小鼠MetS的潜在作用，单独及联合作用，重点关注对肠道微生物群和代谢物谱的重塑作用。在该研究中，L.果中的多酚和多糖改善了MetS的传统药效学参数，包括体重（BW）减轻和脂肪堆积减少、胰岛素抵抗改善、血脂异常调节、肝、肾和近端结肠组织病理变化的预防以及对氧化应激的抑制作用。特别是，联合使用多酚和多糖治疗的组显示出更好的疗效。有益细菌属的相对丰度不同程度增加，而诸如、、和等机会致病菌在治疗后明显减少。此外，粪便代谢物谱揭示了与L.果中多酚和多糖单独及联合治疗相关的23种差异代谢物。总之，这些结果表明，L.果中的多酚和多糖减轻了HFD喂养小鼠的MetS，并改善了肠道微生物群组成和粪便代谢物谱。
