高脂饮食诱导的肥胖小鼠血清代谢组学分析
Analysis of Serum Metabolomics in Obese Mice Induced by High-Fat Diet.
作者信息
Bao Li, Yang Chunjing, Shi Zhengyuan, Wang Zhanrong, Jiang Dechun
机构信息
Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China.
Beijing Key Laboratory of Biocharacteristic Profiling for Evaluation of Rational Drug Use, Beijing, People's Republic of China.
出版信息
Diabetes Metab Syndr Obes. 2021 Nov 30;14:4671-4678. doi: 10.2147/DMSO.S337979. eCollection 2021.
BACKGROUND
Obesity is a public health problem all over the world, and dietary habits are considered one of the important reasons.
METHODS
In this study, serum metabolites of mice fed a normal or high-fat diet (HFD) were analyzed using UPLC-QTOF-MS.
RESULTS
A significant increase in body weight was noted in HFD mice. The HFD and control groups were significantly different from each other on OPLS-DA scores. The major metabolites contributing to obesity were lipid metabolites (phosphatidylcholines, phosphatidylethanolamine, and lysophosphatidylcholines). In addition, this study revealed that glycerophospholipid metabolism, α-linolenic acid metabolism, and linoleic acid metabolism were related to obesity and obesity-associated diseases.
CONCLUSION
These results can be used to better understand obesity and assess its risk, which will provide new ideas for treatment.
背景
肥胖是一个全球性的公共卫生问题，饮食习惯被认为是重要原因之一。
方法
在本研究中，使用超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-QTOF-MS）分析了喂食正常饮食或高脂饮食（HFD）的小鼠的血清代谢物。
结果
高脂饮食小鼠的体重显著增加。高脂饮食组和对照组在正交偏最小二乘法判别分析（OPLS-DA）得分上有显著差异。导致肥胖的主要代谢物是脂质代谢物（磷脂酰胆碱、磷脂酰乙醇胺和溶血磷脂酰胆碱）。此外，本研究表明甘油磷脂代谢、α-亚麻酸代谢和亚油酸代谢与肥胖及肥胖相关疾病有关。
结论
这些结果可用于更好地理解肥胖并评估其风险，这将为治疗提供新思路。
Zotero 插件