Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Shandong Academy of Grape, Jinan 250100, China.
Int J Mol Sci. 2022 Jun 10;23(12):6509. doi: 10.3390/ijms23126509.
Ribosome biogenesis is tightly associated with plant growth and reproduction. Mutations in genes encoding ribosomal proteins (RPs) or ribosome biogenesis factors (RBFs) generally result in retarded growth and delayed flowering. However, the early-flowering phenotype resulting from the ribosome biogenesis defect is rarely reported. We previously identified that the AAA-ATPase MIDASIN 1 (MDN1) functions as a 60S RBF in . Here, we found that its weak mutant is early-flowering. Transcriptomic analysis showed that the expression of () is down-regulated, while that of some autonomous pathway genes and () is up-regulated in . Phenotypic analysis revealed that the flowering time of is severely delayed by increasing expression, suggesting that the early flowering in is likely associated with the downregulation of . We also found that the photoperiod pathway downstream of () and () might contribute to the early flowering in . Intriguingly, the allele completely blocks the early flowering in . Collectively, our results indicate that the ribosome biogenesis defect elicited by the mutation of MDN1 leads to early flowering by affecting multiple flowering regulation pathways.
核糖体生物发生与植物的生长和繁殖密切相关。编码核糖体蛋白（RPs）或核糖体生物发生因子（RBFs）的基因突变通常导致生长迟缓和开花延迟。然而，由于核糖体生物发生缺陷导致的早花表型很少有报道。我们之前已经确定 AAA-ATPase MIDASIN 1 (MDN1) 在 中作为 60S RBF 发挥作用。在这里，我们发现它的弱突变体 是早花的。转录组分析表明，在 中，（）的表达下调，而一些自主途径基因和（）的表达上调。表型分析表明，通过增加 表达严重延迟了 的开花时间，表明 中的早花可能与 （）的下调有关。我们还发现，（）和（）下游的光周期途径可能导致 在 中的早花。有趣的是， 等位基因完全阻断了 在 中的早花。总之，我们的结果表明，MDN1 突变引起的核糖体生物发生缺陷通过影响多个开花调控途径导致早花。
