Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing 100081, China.
Int J Mol Sci. 2022 Jun 15;23(12):6656. doi: 10.3390/ijms23126656.
Petal color is an important agronomic trait in cabbage ( L. var. ). Although the key gene has been functionally characterized, the underlying molecular regulatory mechanism of petal color formation in cabbage is still unclear. In this study, we applied the transcriptome analysis of yellow petals from the cabbage inbred line YL-1 and white petals from the Chinese kale inbred line A192-1 and the -overexpressing transgenic line YF-2 (YL-1 background), which revealed 1928 DEGs common to both the A192-1 vs. YL-1 and the YL-1 vs. YF-2 comparison groups. One key enzyme-encoding gene, , and two key TF-encoding genes, (WRKY) and (SBP), related to carotenoid biosynthesis were significantly up-regulated in both the A192-1 and YF-2 petals, which was consistent with the expression pattern of . We speculate that these key genes may interact with to jointly regulate carotenoid biosynthesis in cabbage petals. This study provides new insights into the molecular regulatory mechanism underlying petal color formation in cabbage.
花瓣颜色是白菜（ L. var. ）的一个重要农艺性状。虽然关键基因的功能已被阐明，但白菜花瓣颜色形成的潜在分子调控机制仍不清楚。在本研究中，我们对黄色花瓣的转录组进行了分析来自自交系 YL-1 的白菜和白色花瓣来自中国甘蓝自交系 A192-1 和过表达转基因系 YF-2（YL-1 背景），揭示了 A192-1 与 YL-1 和 YL-1 与 YF-2 比较组共有的 1928 个差异表达基因。一个关键酶编码基因， 和两个关键 TF 编码基因， （WRKY）和 （SBP），与类胡萝卜素生物合成有关在 A192-1 和 YF-2 花瓣中均显著上调，这与 的表达模式一致。我们推测这些关键基因可能与 相互作用，共同调节白菜花瓣中的类胡萝卜素生物合成。本研究为白菜花瓣颜色形成的分子调控机制提供了新的见解。
