Erexson G L, Kligerman A D, Allen J W
Mutat Res. 1987 May;178(1):117-22. doi: 10.1016/0027-5107(87)90093-5.
A mouse peripheral blood lymphocyte (PBL) micronucleus (MN) test was developed using a modification of the technique for assessing MN in human PBLs described by Fenech and Morley (1985). Male C57Bl/6 mice (5/dose) were injected i.p. with either 0, 2.5, 5.0, 7.5, or 10.0 mg diaziquone (AZQ)/kg. After 24 h the mice were bled by cardiac puncture, PBLs were isolated on a Ficoll-density gradient and then cultured in RPMI 1640 medium using 8 micrograms phytohemagglutinin/ml. In some cultures cytochalasin B (CYB) was added at 21 h during the medium change to block cytokinesis. In other cultures, CYB was omitted to compare the sensitivity of analyzing MN in binucleate versus unblocked mononucleate cells. All doses of AZQ yielded significant increases in MN-containing binucleated PBLs. The use of CYB in the mouse PBL MN test increased the sensitivity approximately 3-fold. The MN test in mouse PBLs should be useful in comparative cytogenetic studies of mice and humans.
采用对Fenech和Morley（1985年）所述的人类外周血淋巴细胞微核评估技术的改良方法，开展了小鼠外周血淋巴细胞（PBL）微核（MN）试验。雄性C57Bl/6小鼠（每组5只）经腹腔注射0、2.5、5.0、7.5或10.0毫克/千克的重氮醌（AZQ）。24小时后，通过心脏穿刺采集小鼠血液，在Ficoll密度梯度上分离PBL，然后在含有8微克/毫升植物血凝素的RPMI 1640培养基中培养。在一些培养物中，在更换培养基的21小时时添加细胞松弛素B（CYB）以阻断胞质分裂。在其他培养物中，不添加CYB，以比较分析双核细胞与未阻断的单核细胞中微核的敏感性。所有剂量的AZQ均使含微核的双核PBL显著增加。在小鼠PBL微核试验中使用CYB可使敏感性提高约3倍。小鼠PBL微核试验在小鼠和人类的比较细胞遗传学研究中应会有用。
