Kligerman A D, Bryant M F, Erexson G L, Rabinowitz J R
Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Environ Mol Mutagen. 1988;12(2):185-99. doi: 10.1002/em.2860120205.
Male C57B1/6 mice were injected i.p. with either 1.25 or 5.0 mg/kg diaziquone (AZQ) and killed at various time intervals from 1 to 99 days post treatment for examination of sister chromatid exchange (SCE) persistence in the peripheral blood lymphocytes (PBLs) and splenocytes. SCE frequencies were found to decay steeply during the first week after exposure in both PBLs and splenocytes. This pattern was followed by a slower decline to baseline over the next week. However, high-frequency cell (HFC) analysis indicates that significant numbers of HFCs persist in the PBLs through day 28 and splenocytes at day 99 post exposure. Mathematical modeling of the time-response curves indicates that the average life span of the majority of AZQ-induced SCE-producing lesions in murine PBLs and splenocytes responsive to phytohemagglutinin is between 3 and 5 days.
给雄性C57B1/6小鼠腹腔注射1.25或5.0毫克/千克的重氮醌（AZQ），并在治疗后1至99天的不同时间间隔处死，以检测外周血淋巴细胞（PBLs）和脾细胞中姐妹染色单体交换（SCE）的持续情况。发现在暴露后的第一周，PBLs和脾细胞中的SCE频率急剧下降。在接下来的一周内，这种模式之后是缓慢下降至基线水平。然而，高频细胞（HFC）分析表明，大量的HFCs在暴露后第28天仍存在于PBLs中，在第99天仍存在于脾细胞中。时间反应曲线的数学模型表明，在对植物血凝素产生反应的小鼠PBLs和脾细胞中，大多数由AZQ诱导产生SCE的损伤的平均寿命在3至5天之间。
