Genome-Wide Identification of the Salvia miltiorrhiza SmCIPK Gene Family and Revealing the Salt Resistance Characteristic of SmCIPK13.

Members of the CIPK (CBL-interacting protein kinases) gene family play important roles in calcium (Ca) signaling pathway-regulated plant resistance to abiotic stresses. , which is widely planted and grown in complex and diverse environments, is mainly focused on the transcriptional regulation of enzyme genes related to the biosynthesis of its bioactive components. However, the excavation of the genes related to the resistance of and the involved signaling pathways have not been deeply studied. In this study, 20 genes were identified and classified into two families and five subfamilies by biochemical means. Sequence characteristics and conserved motif analysis revealed the conservation and difference of SmCIPK protein in plants. Expression pattern analysis showed that were mainly expressed in flowers and roots, and more than 90% of gene expression was induced by SA (salicylic acid), and MeJA (methyl jasmonate). Furthermore, the expression level of could be significantly increased after stress treatment with NaCl. expression in yeast reduces sensitivity to salt, while overexpression of it in has the same effect and was localized in the cytoplasm, cell membrane and nucleus. In conclusion, the identification of the gene family and the functional characterization of the gene provides the basis for clarification of key genes in the Ca signaling pathway and abiotic stress in .