College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Int J Mol Sci. 2022 Jun 20;23(12):6861. doi: 10.3390/ijms23126861.
Members of the CIPK (CBL-interacting protein kinases) gene family play important roles in calcium (Ca) signaling pathway-regulated plant resistance to abiotic stresses. , which is widely planted and grown in complex and diverse environments, is mainly focused on the transcriptional regulation of enzyme genes related to the biosynthesis of its bioactive components. However, the excavation of the genes related to the resistance of and the involved signaling pathways have not been deeply studied. In this study, 20 genes were identified and classified into two families and five subfamilies by biochemical means. Sequence characteristics and conserved motif analysis revealed the conservation and difference of SmCIPK protein in plants. Expression pattern analysis showed that were mainly expressed in flowers and roots, and more than 90% of gene expression was induced by SA (salicylic acid), and MeJA (methyl jasmonate). Furthermore, the expression level of could be significantly increased after stress treatment with NaCl. expression in yeast reduces sensitivity to salt, while overexpression of it in has the same effect and was localized in the cytoplasm, cell membrane and nucleus. In conclusion, the identification of the gene family and the functional characterization of the gene provides the basis for clarification of key genes in the Ca signaling pathway and abiotic stress in .
CIPK（CBL 相互作用蛋白激酶）基因家族成员在钙（Ca）信号通路调控植物抗非生物胁迫中发挥重要作用。, 广泛种植和生长在复杂多样的环境中，主要集中在与其生物活性成分生物合成相关的酶基因的转录调控上。然而，对 抗性相关基因及其参与的信号通路的挖掘尚未深入研究。在这项研究中，通过生化手段鉴定了 20 个基因，并将其分为两个家族和五个亚家族。序列特征和保守基序分析揭示了 SmCIPK 蛋白在植物中的保守性和差异。表达模式分析表明， 主要在花和根中表达，超过 90%的基因表达受 SA（水杨酸）和 MeJA（茉莉酸甲酯）诱导。此外，胁迫处理后 NaCl 可显著提高 的表达水平。 在酵母中的表达降低了对盐的敏感性，而在 中的过表达也有同样的效果，并定位于细胞质、细胞膜和细胞核。综上所述， 基因家族的鉴定和 基因的功能表征为阐明 Ca 信号通路和 中的非生物胁迫关键基因提供了基础。
