Tarkowska Agata, Furmaga-Jabłońska Wanda, Bogucki Jacek, Kocki Janusz, Pluta Ryszard
Department of Neonate and Infant Pathology, Medical University of Lublin, 20-093 Lublin, Poland.
Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Lublin, 20-093 Lublin, Poland.
J Clin Med. 2022 Jun 7;11(12):3263. doi: 10.3390/jcm11123263.
In this study, the expression of the genes of the , , and by RT-PCR in the lymphocytes of newborns after perinatal asphyxia and perinatal asphyxia treated with hypothermia was analyzed at the age of 15-21 days. The relative quantification of Alzheimer's-disease-related genes was first performed by comparing the peripheral lymphocytes of non-asphyxia control versus those with asphyxia or asphyxia with hypothermia. In the newborns who had perinatal asphyxia, the peripheral lymphocytes presented a decreased expression of the and genes. On the other hand, the expression of the and genes increased in the studied group. The expression of the studied genes in the asphyxia group treated with hypothermia had an identical pattern of changes that were not statistically significant to the asphyxia group. This suggests that the expression of the genes involved in the metabolism of the amyloid protein precursor in the peripheral lymphocytes may be a biomarker of progressive pathological processes in the brain after asphyxia that are not affected by hypothermia. These are the first data in the world showing the role of hypothermia in the gene changes associated with Alzheimer's disease in the peripheral lymphocytes of newborns after asphyxia.
在本研究中，于出生后15 - 21天，通过逆转录聚合酶链反应（RT-PCR）分析围产期窒息新生儿及接受低温治疗的围产期窒息新生儿淋巴细胞中、、和基因的表达。首先通过比较非窒息对照组与窒息或窒息伴低温治疗组的外周淋巴细胞，对阿尔茨海默病相关基因进行相对定量分析。在患有围产期窒息的新生儿中，外周淋巴细胞中及基因的表达降低。另一方面，研究组中及基因的表达增加。低温治疗的窒息组中所研究基因的表达变化模式与窒息组相同，且无统计学意义。这表明外周淋巴细胞中淀粉样蛋白前体代谢相关基因的表达可能是窒息后大脑中进行性病理过程的生物标志物，且不受低温影响。这些是世界上首次表明低温在窒息后新生儿外周淋巴细胞中与阿尔茨海默病相关基因变化中的作用的数据。
