一种用于新型隐球菌鉴别的简化埃塞俄比亚芥籽培养基的评估
Evaluation of a simplified Guizotia abyssinica seed medium for differentiation of Cryptococcus neoformans.
作者信息
Paliwal D K, Randhawa H S
出版信息
J Clin Microbiol. 1978 Apr;7(4):346-8. doi: 10.1128/jcm.7.4.346-348.1978.
Abstract
A simplified Guizotia abyssinica seed medium, eliminating glucose, creatinine, and phosphate, was evaluated for the isolation and presumptive identification of Cryptococcus neoformans. Of 80 isolates of C. neoformans tested, 69 (86%) developed the characteristic brown pigment within 12 h on this medium as against only 5 isolates (6%) on the complete medium. In primary cultures of experimentally seeded specimens of sputum, bronchial aspirate, soil, and pigeon excreta, C. neoformans was recognizable within 3 to 5 days on the modified medium in contrast to the 3 to 8 days required on the complete medium. The results demonstrated that the simplified G. abyssinica seed agar, with or without diphenyl, is superior to the complete medium for the rapid development of the brown pigment by C. neoformans.
摘要
对一种简化的埃塞俄比亚芥籽培养基进行了评估，该培养基不含葡萄糖、肌酐和磷酸盐，用于新型隐球菌的分离和初步鉴定。在测试的80株新型隐球菌分离株中，69株（86%）在此培养基上12小时内产生了特征性棕色色素，而在完全培养基上只有5株（6%）产生。在痰液、支气管吸出物、土壤和鸽粪的实验接种标本的原代培养中，新型隐球菌在改良培养基上3至5天内即可识别，而在完全培养基上则需要3至8天。结果表明，无论有无二苯，简化的埃塞俄比亚芥籽琼脂培养基在新型隐球菌快速产生棕色色素方面优于完全培养基。
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Evaluation of a simplified Guizotia abyssinica seed medium for differentiation of Cryptococcus neoformans.一种用于新型隐球菌鉴别的简化埃塞俄比亚芥籽培养基的评估
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本文引用的文献
1
[MEMBRANE FILTRATION AND GUIZOTIA ABYSSINICA CULTURE MEDIA FOR THE DEMONSTRATION OF CRYPTOCOCCUS NEOFORMANS (BROWN COLOR EFFECT)].[用于新型隐球菌（棕色效应）检测的膜过滤法与埃塞俄比亚芥培养基]
Z Hyg Infektionskr. 1963 Oct 25;149:329-36.
2
[A new selective medium for the isolation of C. neoformans from fecal material and from soil].[一种用于从粪便和土壤中分离新型隐球菌的新型选择性培养基]
Ann Inst Pasteur (Paris). 1966 May;110(5):792-3.
3
Medium for selective isolation of Cryptococcus neoformans.新型隐球菌的选择性分离培养基。
Science. 1966 Jan 14;151(3707):208-9. doi: 10.1126/science.151.3707.208.
4
[Selective cultivation of Cryptococcus neoformans].
Mykosen. 1968 Apr 1;11(4):267-72.
5
Identification of Cryptococcus neoformans in a routine clinical laboratory.在常规临床实验室中新型隐球菌的鉴定。
Mycopathol Mycol Appl. 1968 Oct 14;35(3):256-64. doi: 10.1007/BF02050740.
6
Observations on the occurrence of Cryptococcus neoformans in an aviary, using niger-seed creatinine agar and membrane-filtration technique.
Zentralbl Bakteriol Parasitenkd Infektionskr Hyg. 1973 May;128(7):795-9. doi: 10.1016/s0044-4057(73)80103-0.
7
[Cryptococcosis. A contribution to the identification of Cryptococcus neoformans isolated from clinical specimens (author's transl)].[隐球菌病。对从临床标本中分离出的新型隐球菌鉴定的一项贡献（作者译）]
Zentralbl Bakteriol Orig A. 1973 Dec;225(2):211-22.
8
Cryptococcus neoformans of saprophytic origin.源自腐生菌的新型隐球菌。
Sabouraudia. 1975 Nov;13(3):303-8.
9
Occurrence and significance of Cryptococcus neoformans in the oropharynx and on the skin of a healthy human population.健康人群口咽及皮肤中新型隐球菌的发生情况及意义
J Clin Microbiol. 1977 Oct;6(4):325-7. doi: 10.1128/jcm.6.4.325-327.1977.
10
Occurrence and significance of Cryptococcus neoformans in the respiratory tract of patients with bronchopulmonary disorders.支气管肺疾病患者呼吸道中新型隐球菌的发生情况及意义。
J Clin Microbiol. 1977 Jan;5(1):5-8. doi: 10.1128/jcm.5.1.5-8.1977.
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