一种用于新型隐球菌鉴别的简化埃塞俄比亚芥籽培养基的评估
Evaluation of a simplified Guizotia abyssinica seed medium for differentiation of Cryptococcus neoformans.
作者信息
Paliwal D K, Randhawa H S
出版信息
J Clin Microbiol. 1978 Apr;7(4):346-8. doi: 10.1128/jcm.7.4.346-348.1978.
Abstract
A simplified Guizotia abyssinica seed medium, eliminating glucose, creatinine, and phosphate, was evaluated for the isolation and presumptive identification of Cryptococcus neoformans. Of 80 isolates of C. neoformans tested, 69 (86%) developed the characteristic brown pigment within 12 h on this medium as against only 5 isolates (6%) on the complete medium. In primary cultures of experimentally seeded specimens of sputum, bronchial aspirate, soil, and pigeon excreta, C. neoformans was recognizable within 3 to 5 days on the modified medium in contrast to the 3 to 8 days required on the complete medium. The results demonstrated that the simplified G. abyssinica seed agar, with or without diphenyl, is superior to the complete medium for the rapid development of the brown pigment by C. neoformans.
摘要
对一种简化的埃塞俄比亚芥籽培养基进行了评估，该培养基不含葡萄糖、肌酐和磷酸盐，用于新型隐球菌的分离和初步鉴定。在测试的80株新型隐球菌分离株中，69株（86%）在此培养基上12小时内产生了特征性棕色色素，而在完全培养基上只有5株（6%）产生。在痰液、支气管吸出物、土壤和鸽粪的实验接种标本的原代培养中，新型隐球菌在改良培养基上3至5天内即可识别，而在完全培养基上则需要3至8天。结果表明，无论有无二苯，简化的埃塞俄比亚芥籽琼脂培养基在新型隐球菌快速产生棕色色素方面优于完全培养基。
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