两种用于新型隐球菌鉴定的快速色素沉着试验。
Two rapid pigmentation tests for identification of Cryptococcus neoformans.
作者信息
Kaufmann C S, Merz W G
出版信息
J Clin Microbiol. 1982 Feb;15(2):339-41. doi: 10.1128/jcm.15.2.339-341.1982.
Abstract
Two tests were developed for the rapid identification of Cryptococcus neoformans based on pigment produced by the organism's phenoloxidase activity. Caffeic acid was incorporated into cornmeal agar, a medium used routinely for yeast identification. When tested on this medium, only C. neoformans isolates produced brown pigment. All other yeasts maintained their normal morphology and did not produce the reaction product. A non-medium-based test was developed for same-day identification of C. neoformans isolates. Paper strips saturated with a buffered L-beta-3,4-dihydroxyphenylalanine-ferric citrate solution were inoculated with isolates and incubated at 37 degrees C. Pigment production occurred only with C. neoformans isolates, many within 60 to 90 min. All other yeasts remained negative.
摘要
基于新型隐球菌的酚氧化酶活性产生的色素，开发了两种用于快速鉴定新型隐球菌的检测方法。将咖啡酸加入玉米粉琼脂中，玉米粉琼脂是常用于酵母鉴定的培养基。在此培养基上进行检测时，只有新型隐球菌分离株产生棕色色素。所有其他酵母保持其正常形态，不产生反应产物。为了当天鉴定新型隐球菌分离株，开发了一种非基于培养基的检测方法。用缓冲的L-β-3,4-二羟基苯丙氨酸-柠檬酸铁溶液饱和的纸条接种分离株，并在37℃下孵育。只有新型隐球菌分离株产生色素，许多在60至90分钟内产生。所有其他酵母仍为阴性。
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本文引用的文献
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J Clin Microbiol. 1980 Sep;12(3):332-5. doi: 10.1128/jcm.12.3.332-335.1980.
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Pigment formation for differentiating Cryptococcus neoformans from Candida albicans.用于区分新型隐球菌和白色念珠菌的色素形成。
Appl Microbiol. 1971 Mar;21(3):541-2. doi: 10.1128/am.21.3.541-542.1971.
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Pigment production of Cryptococcus neoformans grown with extracts of Guizotia abyssinica.用阿比西尼亚帚菊提取物培养新型隐球菌的色素生成
Appl Microbiol. 1971 Sep;22(3):478-9. doi: 10.1128/am.22.3.478-479.1971.
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Production of diagnostic pigment by phenoloxidase activity of cryptococcus neoformans.新型隐球菌酚氧化酶活性产生诊断色素。
Appl Microbiol. 1972 Nov;24(5):824-30. doi: 10.1128/am.24.5.824-830.1972.
5
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6
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