Bousselham Meriam, Lemriss Sanaa, Dhiba Driss, Aallam Yassine, Souiri Amal, Abbas Younes, Saïdi Nezha, Boukcim Hassan, Hamdali Hanane
Laboratory of Agro-Industrial and Medical Biotechnologies, Faculty of Sciences and Technology, University of Sultan Moulay Slimane, Mghila BP 523, Beni-Mellal 23000, Morocco.
Laboratory of Research and Medical Analysis of Gendarmerie Royale, Department of Biosafety PCL3, Rabat 10090, Morocco.
Microorganisms. 2022 May 28;10(6):1116. doi: 10.3390/microorganisms10061116.
Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four different rhizospheric Moroccan oat soils and to investigate their potential for P solubilization. The distribution of actinomycetes was significantly more abundant in Settat (9.68%), Tangier (7.38%), and Beni Mellal (6.87%) than in the Merchouch-Rabat (4.90%) region. A total of 235 actinomycete strains were isolated from all sites and tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble natural rock phosphate (RP) or synthetic tricalcium phosphate (TCP) as the unique P source. One hundred forty-three isolates (60.8%) had the ability to grow in the SMM with RP whereas only twenty-five isolates (17%) had the most active growth using the SMM with TCP. Eight isolates with the most active growth in solid SMM were selected for their P solubilization abilities in liquid SMM cultures. The highest amount of P solubilized was 163.8 µg/mL for RP and 110.27 µg/mL for TCP after 5 days of culture. The biosolubilization process of AM2, the most efficient RP and TCP solubilizing strain, probably implied the highest excretion of siderophore substances. Eight of these strains were shown to belong to the genus and one to the genus. These findings bolster the phosphate biosolubilization abilities of actinomycetes and may participate in increasing agricultural yields in an eco-efficient and environmentally friendly manner.
土壤放线菌探索似乎是一种有效的生物肥料替代品，可优化磷（P）资源的利用并促进植物生长。本研究旨在探索从摩洛哥四种不同燕麦根际土壤中分离出的放线菌的分布，并研究它们的解磷潜力。塞塔特（9.68%）、丹吉尔（7.38%）和贝尼迈勒（6.87%）的放线菌分布明显比梅尔舒克-拉巴特地区（4.90%）更为丰富。从所有地点共分离出235株放线菌菌株，并测试它们在以不溶性天然磷矿石（RP）或合成磷酸三钙（TCP）作为唯一磷源的合成基本培养基（SMM）上生长的能力。143株分离物（60.8%）有能力在含有RP的SMM中生长，而只有25株分离物（17%）在使用含有TCP的SMM时生长最为活跃。选择在固体SMM中生长最活跃的8株分离物，测试它们在液体SMM培养物中的解磷能力。培养5天后，RP的最高解磷量为163.8 µg/mL，TCP的最高解磷量为110.27 µg/mL。AM2是最有效的RP和TCP溶解菌株，其生物溶解过程可能意味着铁载体物质的排泄量最高。这些菌株中有8株属于 属，1株属于 属。这些发现支持了放线菌的磷酸盐生物溶解能力，并可能以生态高效和环境友好的方式参与提高农业产量。
