Chouyia Fatima Ezzahra, Romano Ida, Fechtali Taoufiq, Fagnano Massimo, Fiorentino Nunzio, Visconti Donato, Idbella Mohamed, Ventorino Valeria, Pepe Olimpia
Department of Biology, Faculty of Sciences and Techniques, Hassan II University, Casablanca, Morocco.
Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy.
Front Plant Sci. 2020 Aug 7;11:1137. doi: 10.3389/fpls.2020.01137. eCollection 2020.
Phosphate-solubilizing bacteria (PSB) have been reported to increase phosphate (P) content and plant growth. Their application in agricultural systems is an eco-friendly alternative strategy for limiting negative environmental impact of chemical fertilizers and increasing costs. Therefore, the aim of this study was to isolate and characterize new putative PSB to use as inoculum to enhance plant growth and increase P bioavailability in soil. Sixteen bacteria were isolated from Moroccan oat rhizosphere and were screened for their putative P-solubilization by semi-quantitative agar spot method. The two strains MS1B15 and MS1B13, identified as and , respectively, showed the maximum phosphate solubilization index (PSI = 1.75 and PSI = 1.63). After quantitative assay to determine phosphate solubilization activity, MS1B15 was selected for evaluating its putative plant growth promotion activities including production of siderophores, indole-3-acetic acid (IAA) and amino-cyclopropane-1-carboxylate (ACC) deaminase, nitrogen fixation and antimicrobial activity against soil-borne plant pathogens. Under greenhouse condition, barley plants inoculated with MS1B15 significantly increased shoot and ear length as well as available phosphorus in ears and leaves and P and N contents in the soil. Overall results showed that the selected strain MS1B15 could represent a potential candidate as biofertilizer to increase plant growth as well as P uptake.
据报道，解磷细菌（PSB）可提高磷（P）含量并促进植物生长。它们在农业系统中的应用是一种生态友好型替代策略，有助于减少化肥对环境的负面影响并降低成本。因此，本研究的目的是分离和鉴定新的潜在解磷细菌，用作接种剂以促进植物生长并提高土壤中磷的生物有效性。从摩洛哥燕麦根际分离出16株细菌，并通过半定量琼脂斑点法筛选它们的潜在解磷能力。两株菌株MS1B15和MS1B13，分别鉴定为 和 ，表现出最大的解磷指数（PSI = 1.75和PSI = 1.63）。在进行定量测定以确定解磷活性后，选择MS1B15评估其潜在的植物生长促进活性，包括铁载体、吲哚-3-乙酸（IAA）和氨基环丙烷-1-羧酸（ACC）脱氨酶的产生、固氮以及对土传植物病原体的抗菌活性。在温室条件下，接种MS1B15的大麦植株显著增加了茎和穗的长度，以及穗和叶片中的有效磷含量以及土壤中的磷和氮含量。总体结果表明，所选菌株MS1B15可能是一种潜在的生物肥料候选物，可促进植物生长并提高磷的吸收。
