Santos Ingrid Nayara Marcelino, Kurihara Mariana Neri Lucas, Santos Fernanda Fernandes, Valiatti Tiago Barcelos, Silva Juliana Thalita Paulino da, Pignatari Antônio Carlos Campos, Salles Mauro José
Laboratório Especial de Microbiologia (LEMC), Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo 04025-010, Brazil.
Laboratório Alerta, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo 04039-032, Brazil.
Microorganisms. 2022 Jun 2;10(6):1149. doi: 10.3390/microorganisms10061149.
spp. remain the leading biofilm-forming agents causing orthopedic implant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of and coagulase-negative (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibiotic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), , virulence and resistance genes were assessed by whole-genome sequencing (WGS). All harbored , , and multiple resistance genes for aminoglycosides and quinolones. All MRSA were strong biofilm producers harboring the complete ADBC and R operon. Seven CoNS isolates comprising five species (, , , and ) were analyzed, with detected in five isolates. (isolate 95), and were unable to form biofilm and did not harbor the complete operon. High variability of adhesion genes was detected, with , , ADBC , and 256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for biofilm production, and resistance to β-lactam and aminoglycosides are associated with treatment failure in OIAIs.
葡萄球菌仍然是导致骨科植入物相关感染（OIAI）的主要生物膜形成病原体。这是一项描述性研究，针对从进展为治疗失败的OIAI患者中分离出的金黄色葡萄球菌和凝固酶阴性葡萄球菌（CoNS）临床菌株所鉴定出的表型和基因组特征进行研究。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定出10株菌株，并对其进行抗生素敏感性和生物膜形成检测。通过全基因组测序（WGS）评估包括多位点序列分型（MLST）、葡萄球菌蛋白A、毒力和耐药基因在内的基因型特征。所有金黄色葡萄球菌均携带葡萄球菌蛋白A、葡萄球菌蛋白A基因盒，以及针对氨基糖苷类和喹诺酮类的多个耐药基因。所有耐甲氧西林金黄色葡萄球菌（MRSA）都是强大的生物膜生产者，携带完整的ADBC和R操纵子。分析了7株CoNS分离株，包括5个菌种（表皮葡萄球菌、溶血葡萄球菌、人葡萄球菌、头状葡萄球菌和模仿葡萄球菌），在5株分离株中检测到了葡萄球菌蛋白A。表皮葡萄球菌（分离株95）和模仿葡萄球菌无法形成生物膜，也未携带完整的葡萄球菌蛋白A操纵子。检测到黏附基因的高度变异性，其中葡萄球菌蛋白A、B、C、ADBC和256是最常见的。总之，携带生物膜产生基因以及对β-内酰胺类和氨基糖苷类耐药的MRSA和CoNS分离株与OIAI的治疗失败有关。
