Du Shuai, You Sihan, Jiang Xiaowei, Li Yuyu, Jia Yushan
National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Molecular Nutrition, Ministry of Education, Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Nutrition and Feed Science of Zhejiang Province, Institute of Feed Science, Zhejiang University, Hangzhou 310058, China.
Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, China.
Microorganisms. 2022 Jun 2;10(6):1154. doi: 10.3390/microorganisms10061154.
This study aimed to characterize the fungal diversity and mycotoxin concentrations of native grass hay in various storage periods. In the present study, the native grass hay samples were collected when stored for 0 d (D0 group), 30 d (D30 group), and 150 d (D150 group), respectively. Here, mycotoxin analyses combined with ITS gene sequence were performed to reveal the changes in response to the storage period. There were notable differences in deoxynivalenol and aflatoxin B1 concentrations among the three groups. Compared to the D150 group, the diversity of the fungal community was higher in the D0 and D30 groups, which indicating the diversity was significantly influenced by the storage period. No significant (p > 0.05) difference was observed among the three groups on the dominant phyla. Interestingly, a significant (p < 0.05) difference was also observed in Chactomella and Aspergillus among the three groups, the abundance of the Chactomella was significantly (p < 0.05) decreased and the abundance of Aspergillus was statistically (p < 0.05) increased in the D150 group. Correlation analysis of the association of fungi with mycotoxin could provide a comprehensive understanding of the structure and function of the fungal community. These results indicated that the good practices of storage are essential for the prevention of mycotoxin. The information contained in the present study is vital for the further development of strategies for hay storage with high quality in the harsh Mongolian Plateau ecosystem.
本研究旨在表征不同储存期天然禾本科干草的真菌多样性和霉菌毒素浓度。在本研究中，分别在储存0天（D0组）、30天（D30组）和150天（D150组）时采集天然禾本科干草样本。在此，结合ITS基因序列进行霉菌毒素分析，以揭示其对储存期的响应变化。三组之间脱氧雪腐镰刀菌烯醇和黄曲霉毒素B1浓度存在显著差异。与D150组相比，D0组和D30组的真菌群落多样性更高，这表明多样性受储存期的显著影响。三组在优势门方面未观察到显著（p>0.05）差异。有趣的是，三组之间在毛壳菌属和曲霉属方面也观察到显著（p<0.05）差异，在D150组中，毛壳菌属的丰度显著（p<0.05）降低，曲霉属的丰度在统计学上（p<0.05）增加。真菌与霉菌毒素关联的相关性分析有助于全面了解真菌群落的结构和功能。这些结果表明良好的储存措施对于预防霉菌毒素至关重要。本研究中包含的信息对于在恶劣的蒙古高原生态系统中进一步制定高质量干草储存策略至关重要。
