Key Laboratory of Evaluation and Application of Changbai Mountain Biological Germplasm Resources of Jilin Province, College of Life Science, Tonghua Normal University, Tonghua, 134002, China.
BMC Genom Data. 2023 Sep 14;24(1):51. doi: 10.1186/s12863-023-01154-1.
Panax ginseng cultivated under the forest is popular because its shape and effective ingredients are similar to wild ginseng. The growth of P. ginseng in the larch forest is generally better than in the broad-leaved forest, and the incidence rate of diseases is low. Therefore, the selection of forest species is one of the basic factors in the successful cropping of P. ginseng.
Illumina HiSeq high-throughput sequencing was used to analyze the 16S rRNA/ITS gene sequence of P. ginseng rhizosphere soil under larch forest to study the rhizosphere microbiome's diversity and community composition structure.
The species classification and richness of rhizosphere bacterial and fungal communities in the same-aged P. ginseng were similar. Consistent with the soil system of commonly cultivated crops, Proteobacteria, Actinobacteriota, Acidobacteriota, Verrucomicrobiota, Chloroflexi, and Basidiomycota, Ascomycota were the dominant phylum of bacteria and fungi, respectively. Compared with the soil without planting P. ginseng, the diversity of microorganisms and community structure of continuous planting for 2 years, 5 years, and 18 years of P. ginseng rhizosphere soil had little change. The accumulation levels of Ilyonectria, Fusarium, Gibberella, and Cylindrocarpon were not significantly increased with planting P. ginseng and the increased age of cropping P. ginseng.
The results of this study showed that the soil function of the larch forest was good, which provided a theoretical basis for the land selection and soil improvement of cultivating P. ginseng under the larch forest.
林下种植的人参因其形状和有效成分与野山参相似而广受欢迎。与阔叶林中相比，人参在落叶松林下的生长通常更好，发病率较低。因此，选择造林树种是人参成功种植的基本因素之一。
采用 Illumina HiSeq 高通量测序技术分析落叶松林下人参根际土壤的 16S rRNA/ITS 基因序列，研究根际微生物群落的多样性和群落组成结构。
同一年龄人参根际细菌和真菌群落的物种分类和丰富度相似。与常见栽培作物的土壤系统一致，变形菌门、放线菌门、酸杆菌门、疣微菌门、绿弯菌门和担子菌门、子囊菌门分别是细菌和真菌的优势门。与未种植人参的土壤相比，连续种植 2 年、5 年和 18 年的人参根际土壤微生物多样性和群落结构变化不大。与种植人参和增加人参种植年龄相对应的是，疫霉属、镰刀菌属、赤霉属和密旋菌属的积累水平并没有显著增加。
本研究结果表明，落叶松林下的土壤功能良好，为林下种植人参的土地选择和土壤改良提供了理论依据。
